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CD62L (L-Selectin) Monoclonal Antibody (MEL-14), eFluor™ 450, eBioscience™
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FIGURE: 1 / 68
CD62L (L-Selectin) Antibody (48-0621-80) in Flow
Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or Anti-Mouse CD62L (L-Selectin) eFluor® 450 (right). Total viable cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
48-0621-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MEL-14
Conjugate
eFluor™ 450
eFluor™ 450
eFluor™ 450
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.
Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD62 antigen-like family member L; hLHRc; Leu-8; leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 1; lymph node homing receptor; lymphocyte antigen 22; lymphocyte surface MEL-14 antigen; unnamed protein product
Gene Aliases: CD62L; L-selectin; LAM-1; LECAM-1; LECAM1; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1
UniProt ID: (Mouse) P18337
Entrez Gene ID: (Mouse) 20343
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
response to ATP
response to cytokine
heterotypic cell-cell adhesion
regulation of apoptotic process
positive regulation of vascular permeability
leukocyte migration
leukocyte tethering or rolling
positive regulation of neutrophil mediated cytotoxicity
positive regulation of neutrophil chemotaxis
negative regulation of leukocyte tethering or rolling
positive regulation of leukocyte adhesion to arterial endothelial cell
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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