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Invitrogen
H3K27me3S28ph Polyclonal Antibody
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FIGURE: 1 / 6
H3K27me3S28ph Antibody (49-1015) in ICC/IF
Asynchronous HeLa cells were formaldehyde fixed, permeabilized with sodium citrate and Triton® X-100 (Dow Chemical Co) and blocked with PBS containing 2.5% BSA. (A) Cells were labeled with the anti-H3K27me3S28p crude serum (diluted 1:200 and incubated for 1 hour at room temperature) followed by labeled goat anti-rabbit secondary antibody (Fisher Scientific). (B) Nuclei were stained using the DNA-specific st... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
49-1015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Raised against histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p), using a KLH conjugated synthetic peptide.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
<0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H3 histone family, member L; histone 1, H3b; histone 2, H3a; histone cluster 1 H3 family member b; histone cluster 1, H3b; histone cluster 2 H3 family member a; histone cluster 2, H3a; histone H3/l; histone H3/o
Gene Aliases: H3/l; H3/n; H3/o; H3FL; HIST1H3B; HIST2H3A
UniProt ID: (Human) Q71DI3, (Human) P68431
Entrez Gene ID: (Human) 333932, (Human) 8358
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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