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Invitrogen
CD34 Monoclonal Antibody (RAM34), eFluor™ 660, eBioscience™
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FIGURE: 1 / 47
CD34 Antibody (50-0341-82) in Flow
Staining of BALB/c bone marrow cells with Anti-Mouse CD117 (c-Kit) FITC (Product # 11-1171-82) and 1.0 µg of Anti-Mouse CD34 eFluor® 660. Lineage negative cells using the Mouse Hematopoietic Lineage eFluor® 450 Cocktail (Product # 88-7772-72) were used for analysis.
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20240507?time=20240507 "CD34 Antibody (50-0341-82) in ICC/IF")
.jpg?time=20240507?time=20240507 "CD34 Antibody (50-0341-82) in Flow")
Product Details
50-0341-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Gerbil,
Goat,
Hamster,
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
RAM34
Conjugate
eFluor™ 660
eFluor™ 660
eFluor™ 660
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.
Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Note: When staining with the RAM34 antibody an incubation time of 90 minutes is recommended to obtain the optimal signal to noise ratio.
For more detailed information on staining with RAM34, please refer to Ema et al., listed under References.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: cell surface antigen; cluster designation 34; Mucosialin; RP11-328D5.2; stem cell antigen; unnamed protein product
UniProt ID: (Mouse) Q64314
Entrez Gene ID: (Mouse) 12490
tissue homeostasis
endothelial cell proliferation
glomerular filtration
endothelium development
cell adhesion
cell-matrix adhesion
signal transduction
positive regulation of gene expression
negative regulation of gene expression
hemopoiesis
negative regulation of interleukin-2 production
mesangial cell-matrix adhesion
paracrine signaling
positive regulation of odontogenesis
positive regulation of angiogenesis
cell motility
leukocyte migration
transdifferentiation
vascular wound healing
hematopoietic stem cell proliferation
positive regulation of granulocyte colony-stimulating factor production
extracellular exosome assembly
glomerular endothelium development
stem cell proliferation
metanephric glomerular mesangial cell differentiation
cell-cell adhesion
positive regulation of vasculogenesis
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