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CD363 (S1PR1) Monoclonal Antibody (SW4GYPP), eFluor™ 660, eBioscience™
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FIGURE: 1 / 2
CD363 (S1PR1) Antibody (50-3639-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0047-42) and Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human CD363 (S1PR1) eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
50-3639-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SW4GYPP
Conjugate
eFluor™ 660
eFluor™ 660
eFluor™ 660
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The SW4GYPP monoclonal antibody recognizes human CD363, also known as sphingosine-1-phosphate receptor 1 (S1PR1, S1P1), or endothelial differentiation G-protein coupled receptor 1 (EDG1). CD363 is a part of a family of five G-protein-coupled receptors that bind sphingosine-1-phosphate (S1P), a lysophospholipid mediator. It has been shown to be primarily expressed by lymphocytes but is also seen on endothelial cells, B cells, NK cells, monocytes/macrophages, neural progenitors, neurons, astrocytes, and oligodendrocytes. CD363 surface expression is dependent on the extracellular concentration of S1P and its signaling is important for lymphocyte egress from secondary lymphoid tissues and the thymus. CD363 plays a key role in many cellular processes like angiogenesis, vascular maturation, endothelial barrier function and trafficking of immune cells. Therapeutic CD363 modulators have been successful in the treatment of relapsing-remitting multiple sclerosis through sequestration of immune cells in secondary lymphoid organs and, therefore, away from sites of inflammation.
Applications Reported: This SW4GYPP antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SW4GYPP antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD antigen CD363; CD363 antigen; EDG1; endothelial differentiation G-protein coupled receptor 1; endothelial differentiation protein (edg-1); endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; S1P receptor Edg-1; similar to spleen endothelial differentiation gene EDG-1; sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1; unnamed protein product
Gene Aliases: CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; S1P1
UniProt ID: (Human) P21453
Entrez Gene ID: (Human) 1901
angiogenesis
blood vessel maturation
cardiac muscle tissue growth involved in heart morphogenesis
sphingosine-1-phosphate signaling pathway
chemotaxis
cell adhesion
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
cell migration
transmission of nerve impulse
lamellipodium assembly
actin cytoskeleton organization
neuron differentiation
positive regulation of cell migration
regulation of bone mineralization
regulation of bone resorption
endothelial cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of positive chemotaxis
negative regulation of stress fiber assembly
heart trabecula morphogenesis
T cell migration
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