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Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eFluor™ 660, eBioscience™
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FIGURE: 1 / 38
Podoplanin Antibody (50-5381-80) in IHC (P)
Immunohistochemical analysis of Podoplanin was performed using formalin-fixed paraffin-embedded mouse lung and mouse skeletal muscle tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degre... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
50-5381-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Syrian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
eBio8.1.1 (8.1.1)
Conjugate
eFluor™ 660
eFluor™ 660
eFluor™ 660
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.
Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aggrus; CTA-520D8.1; glycoprotein 38; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; RP23-348F1.2; transmembrane glycoprotein E11; unnamed protein product
Gene Aliases: E11; Gp38; OTS-8; RANDAM-2; T1-alpha; T1a; T1alpha
UniProt ID: (Mouse) Q62011
Entrez Gene ID: (Mouse) 14726
lymphangiogenesis
prostaglandin metabolic process
amino acid transport
cell adhesion
signal transduction
Rho protein signal transduction
cell proliferation
negative regulation of cell proliferation
positive regulation of platelet activation
positive regulation of epithelial to mesenchymal transition
folic acid transport
cell migration
regulation of cell adhesion
lung development
positive regulation of cell migration
tube morphogenesis
negative regulation of apoptotic process
wound healing, spreading of cells
lung alveolus development
lymph node development
lymphatic endothelial cell fate commitment
visceral serous pericardium development
actin-mediated cell contraction
positive regulation of extracellular matrix disassembly
cell-cell adhesion
regulation of substrate adhesion-dependent cell spreading
positive regulation of platelet aggregation
regulation of myofibroblast contraction
regulation of G1/S transition of mitotic cell cycle
positive regulation of cell motility
regulation of lamellipodium morphogenesis
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