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Invitrogen
Pdx1 Monoclonal Antibody (2A12), eFluor™ 660, eBioscience™
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Product Details
50-6500-82
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2A12
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This 2A12 monoclonal antibody reacts with the transcriptional activator PDX1, also known as pancreatic and duodenal homeobox protein and insulin promoter factor. Early expression of PDX1 during development marks the endoderm from which the pancreas, stomach, and duodenum derive. In the mature pancreas, expression is found in insulin-expressing beta-cells. PDX1 functions as an important regulator of several key pancreatic proteins including insulin, somatostatin, glucokinase, islet amyloid factor, and glucose transporter type 2. Deficiency in PDX1 results in a dysregulation of the beta-cell compensation of insulin resistance, which can lead to late-onset type 2 diabetes mellitus (formerly NIDDM) and maturity onset diabetes of the young type 4 (MODY4).
Applications Reported: This 2A12 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This 2A12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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