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CXCL1 (GRO alpha) Monoclonal Antibody (W3ZLCXC), eFluor™ 660, eBioscience™
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FIGURE: 1 / 1
CXCL1 (GRO alpha) Antibody (50-7519-42) in Flow
Normal human peripheral blood monocytes were unstimulated (left) or stimulated with LPS in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980) (right). Cells were fixed and stained intracellularly with Anti-Human CD14 FITC (cat. 11-0149) and Anti-Human CXCL1 eFluor® 660 using the Fixation and Permeabilization Buffers (cat. 88-8823). Cells in the monocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
50-7519-42
Product Specifications
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
W3ZLCXC
Conjugate
eFluor™ 660
eFluor™ 660
eFluor™ 660
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
CXCL1 is secreted by monocytes, epithelial cells, and fibroblasts in response to pro-inflammatory stimuli such as LPS, IL-1 beta, and TNF alpha. Overexpression is observed in many malignant tumors, where it contributes to tumor vascularization and metastasis. Recent data suggests this antibody has slight crossreactivity by ELISA to CXCL2 and CXCL3. This has not been confirmed by flow cytometry. For a more specific antibody to CXCL1, please use clone KTYFLF (cat 50-7515).
This W3ZLCXC antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
This W3ZLCXC antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of human peripheral blood monocytes. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
eFluor™ 660 is an alternative for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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