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GARP Monoclonal Antibody (G14D9), eFluor™ 660, eBioscience™
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GARP Antibody (50-9882-41) in Flow
Normal human peripheral blood cells were stimulated with immobilized Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81), soluble Anti-Human CD28 Functional Grade Purified (Product # 16-0289-81), and Human IL-2 Recombinant Protein (Product # 14-8029-81) for 24 hours. Cells were surface stained with Anti-Human CD4 PE (Product # 12-0048-42) and Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or Anti-Human GARP eFluor® 660 (right). Cells were then fixed and permeabilized with the Foxp3/Transcription Factor St... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
50-9882-41
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
G14D9
Immunogen
A mixture of mGARP-Fc and hGARP 21H-F1 & -F2
Conjugate
eFluor™ 660
eFluor™ 660
eFluor™ 660
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predominant (GARP, also known as LRRC32 or Garpin). GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface. Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells. The expression of GARP on the surface of activated Treg cells has been reported to be necessary for their suppressive function, possibly related to its role as a cell surface receptor for LAP/TGF beta.
Applications Reported: This G14D9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This G14D9 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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