Search
Invitrogen
IL-13 Polyclonal Antibody, PeproTech®
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 7
IL-13 Antibody (500-P13-50UG) in WB
Western Blot: To detect Human IL-13 by Western Blot analysis IL-13 Polyclonal Antibody (Product # 500-P13-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P13-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-13
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD LLLHLKKLFR EGRFN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-13. Anti-Human IL-13-specific antibody was purified by affinity chromatography employing an immobilized Human IL-13 matrix.
Sandwich ELISA: To detect Human IL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-13 (500-P13Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-13.
Western Blot: To detect Human IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; ILN; Interleukin; Interleukin13; MGC116786; MGC116788; MGC116789; NC30; NC30 antibody; P600 antibody
Gene Aliases: IL-13; P600
UniProt ID: (Human) P35225
Entrez Gene ID: (Human) 3596
microglial cell activation
positive regulation of immunoglobulin production
apoptotic process
inflammatory response
immune response
JAK-STAT cascade
response to mechanical stimulus
regulation of proton transport
positive regulation of gene expression
positive regulation of B cell proliferation
response to lipopolysaccharide
positive regulation of interleukin-10 production
transforming growth factor beta1 production
regulation of transforming growth factor beta1 production
response to nicotine
interleukin-13-mediated signaling pathway
macrophage activation
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of macrophage activation
positive regulation of ion transport
response to ethanol
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of protein secretion
negative regulation of inflammatory response
positive regulation of inflammatory response
positive regulation of release of sequestered calcium ion into cytosol
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to mechanical stimulus
negative regulation of transforming growth factor beta production
positive regulation of cold-induced thermogenesis
negative regulation of lung ciliated cell differentiation
positive regulation of lung goblet cell differentiation
negative regulation of complement-dependent cytotoxicity
positive regulation of pancreatic stellate cell proliferation
negative regulation of endothelial cell apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support