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GPR109 Monoclonal Antibody (4NZBRGO), Alexa Fluor™ 647, eBioscience™
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FIGURE: 1 / 1
GPR109 Antibody (51-4779-42) in Flow
Normal human peripheral blood were stained with Anti-Human CD16 PerCP-eFluor® 710 (Product # 46-0168-42), Anti-Human CCR3 APC (Product # 17-1939-42), and Mouse IgG1 K Isotype Control Alexa Fluor® 647 (blue histogram) or Anti-Human GPR109 Alexa Fluor® 647 (purple histogram). CD16-CCR3+ or CD16+CCR3- (Neutrophils) cells in the granulocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
51-4779-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Bovine
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4NZBRGO
Conjugate
Alexa Fluor™ 647
Alexa Fluor™ 647
Alexa Fluor™ 647
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This 4NZBRGO antibody has not been tested for reactivity with human GPR109B.
Applications Reported: This 4NZBRGO antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 4NZBRGO antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
GPR109A (also known as HCA2, HM74a, and NIACR1) is a 42kDa G-protein coupled receptor that belongs to a family of three hydroxyl-carboxylic acid receptors, which also include GPR109B and GPR81. GPR109A has two known ligands: niacin and butyrate (or its derivative 3-hydroxybutyrate). The endogenous ligands for GPR109B and GPR81 are 3-hydroxyl-octanoic acid and 2-hydroxy-propanoate (lactate), respectively. GPR109A is expressed on adipocytes, neutrophils, Langerhans cells, keratinocytes, intestinal epithelial cells, and intestinal colonic macrophages. Niacin, also known as nicotinic acid or vitamin B3, is known to improve cholesterol metabolism (LDL/HDL ratio) and reduce inflammation. It has been demonstrated that this effect is mediated through GPR109A. Notably, GPR109A also has an essential role in suppressing inflammation and cancer in the colon. Both GPR109A ligands are produced by colonic commensal bacteria. In mice, GPR109A expression on intestinal epithelial cells and DCs/macrophages contributes to the suppression of colitis and colon cancer. In humans, GPR109A is silenced in colon carcinoma cells by DNA methylation, which increases inflammation in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein-coupled receptor HM74a; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; hydroxy-carboxylic acid receptor 2; niacin receptor 1; nicotinic acid receptor; PUMA-G. GPR109; unnamed protein product
Gene Aliases: GPR109A; HCA2; HM74a; HM74b; NIACR1; Puma-g; PUMAG
UniProt ID: (Human) Q8TDS4
Entrez Gene ID: (Human) 338442
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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