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CD204 Monoclonal Antibody (M204PA), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 1
CD204 Antibody (53-2046-82) in Flow
C57BL/6 mouse resident peritoneal exudate cells were stained with F4/80 Monoclonal Antibody, APC (Product # 17-4801-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-82) (left) or 0.125 µg of CD204 Monoclonal Antibody, Alexa Fluor 488 (right). Total cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
53-2046-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
M204PA
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This M204PA antibody recognizes mouse CD204 also known as Macrophage Scavenger Receptor 1 (MSR 1), Scavenger Receptor A (SR-A) or SCARA1. CD204 belongs to the class A family of scavenger receptors that are primarily responsible for binding a wide variety of macromolecules and are involved in many aspects of macrophage activity. Other members of this family include: MARCO (SCARA2), CSR (SCARA3), SRCL (SCARA4) and SCARA5. All class A scavenger receptors are trans-membrane proteins with extracellular C-termini and homotrimeric structures. CD204 exists as 2 splice variants: the more ubiquitous full length isoform (type I) and a shorter isoform (type II) that lacks the C-terminal cysteine-rich domain. Both types are functional and bind modified LDL with similar affinity. In addition to lipid metabolism, CD204 plays an important role suppressing inflammation and macrophage activation. CD204 is considered one of the markers of M2 and Tumor-Associated Macrophages. Interestingly, this molecule is responsible for adherence of RAW264.7 cells to tissue culture-treated plastic in the absence of Ca and Mg cations.
This M204PA antibody binds to CD204 of multiple mouse strains, including C57Bl/6, Balb/c and Swiss-Webster (outbred). This antibody works on fixed cells and can be used to stain CD204 intracellularly. The M204PA antibody does not cross-react with human or rat. This antibody is not suitable for IHC staining.
Applications Reported: This M204PA antibody has been reported for use in flow cytometric analysis.
Applications Tested: This M204PA antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells.This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
CD204 gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Diseases associated with MSR1 include Barrett Esophagus and Prostate Cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: macrophage acetylated LDL receptor I and II; scavenger receptor type A; SR-AI/II; unnamed protein product
Gene Aliases: MRS-A; MSR; MSR-A; Scara1; Scvr; SR-A; SR-AI; SR-AII
UniProt ID: (Mouse) P30204
Entrez Gene ID: (Mouse) 20288
endocytosis
receptor-mediated endocytosis
phagocytosis, engulfment
negative regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol storage
cholesterol transport
positive regulation of toll-like receptor 9 signaling pathway
plasma lipoprotein particle clearance
lipoprotein transport
beta-amyloid clearance
Disclaimer
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