UltraLink™ Iodoacetyl Resin

Catalog number: 53155

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

Thermo Scientific UltraLink Iodoacetyl Resin is a durable, porous resin that is activated to enable efficient and direct covalent immoblization of peptides and other ligands through their sulfhydryl groups (-SH) for use in affinity purification procedures.

Features of UltraLink Iodoacetyl Resin:

High coupling efficiency and capacity—immobilizes sulfhydryl-containing proteins or other ligands with high efficiency and coupling capacity in 1 hour
Specific and leak-proof coupling chemistry—reacts specifically with sulfhydryl groups (reduced thiols), resulting in thioether bonds that are stable for use in many affinity purification procedures
Simple coupling conditions—perform immobilization reaction in any of a variety of buffers; coupling is most efficient and specific at pH 8.0-8.5; coupling compatible with or without organic solvent
Excellent reusability—prepared affinity resin can be used with typical binding and elution procedures for many cycles of affnity purification without significant loss of binding capacity
Durable, high-performance resin—porous beads have a 60 µM diameter, can withstand 100 psi (6.9 bar) and allow for linear flow-through rates of 3,000 cm/hour

The beaded resin is a hydrophilic copolymer of polyacrylamide and azlactone having a rigid polymeric structure with high surface area and pore volume. Each azlactone group has been modified to form a 15-atom spacer arm that terminates in an iodoacetyl group, which is capable of reacting with sulfhydryl groups (e.g., side chain of reduced cysteine residues) to covalently immobilize peptide or other sulfhydryl-containing ligands. The bead structure and efficiently coupling chemistry of Iodoacetyl-Activated UltraLink Support results in high protein binding capacity, high linear flow rates, low nonspecific binding and overall superior performance in affinity chromatography. UltraLink Resins are ideal for medium pressure applications such as FPLC.

Properties of UltraLink Iodoacetyl Resin:
Alternate names: 3M Emphaze Biosupport Medium AB1
Matrix: Polyacrylamide/azlactone copolymer derivatized with iodoacetyl groups
Particle size: 50 to 80 µM diameter
Maximum pressure: 100 psi (6.9 bar)
Maximum linear flow rate: 3000 cm/hour
Coupling capacity: > 6 mg of reduced human IgG per ml of settled resin
Leaving group in coupling reaction: Hydrogen iodide
Bond formed: Thioether bond
Spacer arm: 15 atoms

Related Products
UltraLink™ Iodoacetyl Micro Peptide Coupling Kit, 0.1 mL
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Affinity, UltraLink™ Resin
Format: Bottle(s)
Immobilized Phase: Haloacetyl
Product Size: 10 mL

Contents & storage

Storage: Upon receipt store product at 4°C, protected from light. Product is shipped at ambient temperature

Documents

Manuals & protocols

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