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IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor™ 660, eBioscience™

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Product Details

606-7319-41

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.015 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4S.B3

Conjugate

Alexa Fluor™ 660 Alexa Fluor™ 660 Alexa Fluor™ 660

Excitation/Emission Max

663/691 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2896301

Product Specific Information

Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4S.B3 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.

Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: cytokine; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNg; IFNγ; immune interferon; Interferon; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g; unnamed protein product

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Gene Aliases: IFG; IFI; IMD69

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UniProt ID: (Human) P01579

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Entrez Gene ID: (Human) 3458

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Function(s)
cytokine activity interferon-gamma receptor binding protein binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter microglial cell activation positive regulation of cytokine production adaptive immune response macrophage activation involved in immune response apoptotic process immune response humoral immune response cell surface receptor signaling pathway JAK-STAT cascade positive regulation of cell proliferation response to virus positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration macrophage differentiation negative regulation of epithelial cell differentiation negative regulation of interleukin-17 production positive regulation of chemokine production positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of peptidyl-serine phosphorylation of STAT protein positive regulation of smooth muscle cell apoptotic process Fc-gamma receptor signaling pathway involved in phagocytosis type III interferon-mediated signaling pathway macrophage activation positive regulation of tyrosine phosphorylation of STAT protein positive regulation of MHC class II biosynthetic process positive regulation of nitric oxide biosynthetic process positive regulation of osteoclast differentiation positive regulation of cell adhesion positive regulation of glycolytic process negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter astrocyte activation negative regulation of smooth muscle cell proliferation positive regulation of inflammatory response positive regulation of phagocytosis positive regulation of neurogenesis regulation of insulin secretion positive regulation of membrane protein ectodomain proteolysis interferon-gamma-mediated signaling pathway positive regulation of fructose 1,6-bisphosphate metabolic process positive regulation of vitamin D biosynthetic process extrinsic apoptotic signaling pathway receptor signaling pathway via STAT cellular response to virus neuroinflammatory response negative regulation of beta-amyloid clearance positive regulation of glutamate receptor signaling pathway positive regulation of cellular respiration positive regulation of beta-amyloid formation positive regulation of protein localization to plasma membrane positive regulation of exosomal secretion positive regulation of iron ion import across plasma membrane positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
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