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Invitrogen

ROR gamma (t) Monoclonal Antibody (B2D), PE-eFluor™ 610, eBioscience™

7 Published Figures
20 References
View all (14) ROR gamma (t) antibodies

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Product Details

61-6981-80

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test
View 19 publications 19 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B2D

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574649

Product Specific Information

Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymocytes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

The ROR gamma t isoform differs from ROR gamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms.

Applications Reported: This B2D antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular protein under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

ROR gammat t (RORgt) is an isoform of RORC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: MGC129539; nuclear receptor RZR-gamma; nuclear receptor subfamily 1 group F member 3; orphan nuclear receptor; RAR-related orphan receptor C; retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORgt; RP11-98D18.11-001; thymus orphan receptor; unnamed protein product

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Gene Aliases: Nr1f3; RORgamma; Thor; TOR

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UniProt ID: (Mouse) P51450

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Entrez Gene ID: (Mouse) 19885

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding oxysterol binding zinc ion binding sequence-specific DNA binding transcription factor activity, direct ligand regulated sequence-specific DNA binding sequence-specific double-stranded DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter tolerance induction in gut-associated lymphoid tissue regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation xenobiotic metabolic process regulation of gene expression regulation of glucose metabolic process regulation of steroid metabolic process cell differentiation intracellular receptor signaling pathway circadian regulation of gene expression T cell differentiation in thymus cellular response to sterol T-helper cell differentiation positive regulation of circadian rhythm regulatory T cell differentiation regulation of gamma-delta T cell differentiation regulation of fat cell differentiation positive regulation of transcription, DNA-templated alpha-beta T cell differentiation lymph node development mucosal-associated lymphoid tissue development Peyer's patch development adipose tissue development negative regulation of thymocyte apoptotic process T-helper 17 cell differentiation
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