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Invitrogen
ZO-1 Polyclonal Antibody
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FIGURE: 1 / 99
ZO-1 Antibody (61-7300) in WB
Comparison of western blot performance was done using ZO-1 Polyclonal Antibody (Product # 61-7300) and a top-cited ZO-1 antibody. ZO-1 expression was detected at ~200 kDa in Fig. (a). Caco-2 whole cell extracts loaded at varied concentrations 30 µg (Lane 1), 20 µg (Lane 2), 10 µg (Lane 3) and 5 µg (Lane 4) were electrophoresed using NuPAGE™ 3 to 8%, Tris-Acetate, 1.0 mm, Mini Protein Gel (Product # EC6695BOX), 10-well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB33001) by iBlot™ 3 Western Blot Transf... View More
Product Details
61-7300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Guinea pig,
Human,
Mouse,
Non-human primate,
Rat
Published species
Avian,
Baboon,
Bacteria,
Bovine,
C. elegans,
Cat,
Chicken,
Cynomolgus monkey,
Dog,
Fish,
Fruit fly,
Guinea pig,
Hamster,
Horse,
Human,
Insect,
Mouse,
Non-human primate,
Pig,
Plant,
Rabbit,
Rat,
Rhesus monkey,
Sheep,
Tag,
Virus,
Xenopus,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 69 kD fusion protein(1) corresponding to amino acids 463-1109 of human ZO-1 cDNA.(2) This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
61-7300 has been successfully used in Western blot, Immunoprecipitation, Immunofluorescence, ELISA and Immunohistochemistry.
To achieve appropriate staining results on formalin-fixed, paraffin embedded tissue sections, protease Type XIV (Actinase E) pretreatment is required. The recommended dilution and incubation for protease is 1-2 mg/mL at 37°C for 10 minutes. Other enzyme and Heat Induced Epitope Retrieval pre-treatment methods may not be suitable for this particular antibody. Frozen sections have been successfully stained after fixation with acetone at 4°C.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; unnamed protein product; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Gene Aliases: TJP1; ZO-1; ZO1
UniProt ID: (Human) Q07157, (Dog) O97758, (Mouse) P39447, (Rat) A0A0G2K2P5
Entrez Gene ID: (Human) 7082, (Dog) 403752, (Mouse) 21872, (Rat) 292994, (Guinea pig) 100718142
cell-cell junction assembly
positive regulation of cell proliferation
actin cytoskeleton organization
positive regulation of cell migration
actomyosin structure organization
adherens junction maintenance
maintenance of blood-brain barrier
ameloblast differentiation
negative regulation of apoptotic process
cell-cell junction organization
regulation of cytoskeleton organization
negative regulation of stress fiber assembly
protein localization to adherens junction
establishment of endothelial intestinal barrier
cell-cell adhesion
protein localization to cell-cell junction
regulation of cell junction assembly
protein localization to bicellular tight junction
positive regulation of sprouting angiogenesis
positive regulation of maintenance of permeability of blood-brain barrier
positive regulation of cell-cell adhesion mediated by cadherin
regulation of bicellular tight junction assembly
bicellular tight junction assembly
cellular response to calcium ion
blastocyst formation
sensory perception of sound
response to lipopolysaccharide
response to drug
negative regulation of vascular permeability
response to ethanol
response to magnetism
cellular response to glucose stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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