ERK 1/2 Colorimetric In-Cell ELISA Kit, Multispecies

Catalog number: 62206

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce ERK1/2 Colorimetric In-Cell ELISA Kit is a microplate assays optimized to measure total and phosphorylated extracellular signal-regulated kinase 1 and 2 (phospho-ERK1/2) in whole cells.

Features of the ERK1/2 Colorimetric In-Cell ELISA Kit:

• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative Western blotting
• Less costly (one well vs. one lane) than Western blotting
• Measures total and phosphorylated extracellular signal-regulated kinase 1 and 2 (ERK1/2) in whole cells using a 96-well microplates

This ELISA kit includes a mouse monoclonal anti-ERK1/2 antibody, rabbit monoclonal anti-phospho-(Thr202/Tyr204) ERK1/2 antibody, detection reagents, and all buffers required for colorimetric detection. The ERK1/2 In-Cell ELISA Kit enable simultaneous detection of both total and phospho-(Thr202/Tyr204) ERK1/2.

Overview of ERK1/2 and phosphorylation of ERK1/2
ERK1 and ERK2 are also referred to as mitogen-activated protein kinase 3 and 1, respectively (MAPK3, MAPK1) and regulate a broad range of cellular processes including gene transcription, cytoskeletal organization, metabolic homeostasis, cell growth, and apoptosis. Within the MAPK/ERK signal transduction pathway, extracellular stimuli may be transmitted into the cell through a receptor tyrosine kinase (RTK) and relayed through MAPKK kinase phosphorylation of MAPK kinase which, in turn, phosphorylates MAPK family members. Dual phosphorylation of ERK1/2 on thr 202 and tyr 204 causes translocation into the cell nucleus and phosphorylation of transcriptional regulators. Disregulation of ERK1/2 is implicated in cancer phenotypes. The ERK1/2 In-Cell ELISA Kit enables simultaneous detection of both total and phospho-(Thr202/Tyr204) ERK1/2.

Related Products
Pierce™ ERK1/2 Near Infrared In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Multiple
Product Size: 96 assays
Protein Family: Other Proteins
Detection Method: Colorimetric
Research Category: Signal Transduction

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 50 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• HRP Conjugate, 0.14 mL
• TMB Substrate, 58 mL
• TMB Stop Solution, 55 mL
• Janus Green Whole-Cell Stain, 50 mL
• Elution Buffer, 2 x 30 mL
• Thin Plate Seal Assembly, 8 each
• Anti-Phospho ERK 1/2 (Thr 202/Tyr 204) Antibody
• Anti-ERK1/2 Antibody

Store antibodies as indicated on label. All other kit components should be stored at 4°C.

Documents

Manuals & protocols