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ERK1/ERK2 Multispecies In-Cell ELISA Kit, Colorimetric

Catalog number:  62206

 Related applications: Protein Assays and Analysis

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The Thermo Scientific Pierce ERK1/2 Colorimetric In-Cell ELISA Kit is a microplate assays optimized to measure total and phosphorylated extracellular signal-regulated kinase 1 and 2 (phospho-ERK1/2) in whole cells.

Features of the ERK1/2 Colorimetric In-Cell ELISA Kit:

• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative Western blotting
• Less costly (one well vs. one lane) than Western blotting
• Measures total and phosphorylated extracellular signal-regulated kinase 1 and 2 (ERK1/2) in whole cells using a 96-well microplates

This ELISA kit includes a mouse monoclonal anti-ERK1/2 antibody, rabbit monoclonal anti-phospho-(Thr202/Tyr204) ERK1/2 antibody, detection reagents, and all buffers required for colorimetric detection. The ERK1/2 In-Cell ELISA Kit enable simultaneous detection of both total and phospho-(Thr202/Tyr204) ERK1/2.

Overview of ERK1/2 and phosphorylation of ERK1/2
ERK1 and ERK2 are also referred to as mitogen-activated protein kinase 3 and 1, respectively (MAPK3, MAPK1) and regulate a broad range of cellular processes including gene transcription, cytoskeletal organization, metabolic homeostasis, cell growth, and apoptosis. Within the MAPK/ERK signal transduction pathway, extracellular stimuli may be transmitted into the cell through a receptor tyrosine kinase (RTK) and relayed through MAPKK kinase phosphorylation of MAPK kinase which, in turn, phosphorylates MAPK family members. Dual phosphorylation of ERK1/2 on thr 202 and tyr 204 causes translocation into the cell nucleus and phosphorylation of transcriptional regulators. Disregulation of ERK1/2 is implicated in cancer phenotypes. The ERK1/2 In-Cell ELISA Kit enables simultaneous detection of both total and phospho-(Thr202/Tyr204) ERK1/2.

Related Products
Pierce™ ERK1/2 Near Infrared In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Other Proteins
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
Detector Antibody Conjugate: Unconjugated (uses Secondary Antibody)
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: ERK, ERK-2, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK, ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK
Gene ID: 5594, 5595
Gene Name: mitogen-activated protein kinase 1, mitogen-activated protein kinase 3
Gene Symbol: MAPK1, MAPK3
Immunoassay Kit Format: In-Cell ELISA Kit
Immunoassay Kit Grade: Colorimetric
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3
Target (Kit Named): ERK1/ERK2
Target Species (Named): Multispecies
Target Species (Validated): Human
UniProt ID: P27361, P28482

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 50 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• HRP Conjugate, 0.14 mL
• TMB Substrate, 58 mL
• TMB Stop Solution, 55 mL
• Janus Green Whole-Cell Stain, 50 mL
• Elution Buffer, 2 x 30 mL
• Thin Plate Seal Assembly, 8 each
• Anti-Phospho ERK 1/2 (Thr 202/Tyr 204) Antibody
• Anti-ERK1/2 Antibody

Store antibodies as indicated on label. All other kit components should be stored at 4°C.


Manuals & protocols