Caspase 3 (Cleaved) Multispecies In-Cell ELISA Kit, Colorimetric

Catalog number: 62218

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

This Thermo Scientific Pierce Cleaved Caspase 3 Colorimetric In-Cell ELISA Kit is a colorimetric microplate assays optimized to measure activated (cleaved) caspase 3 and tubulin in whole cells.

This ELISA kit for whole cells includes a rabbit monoclonal anti-(cleaved) Caspase antibody, a mouse monoclonal anti-alpha-tubulin antibody, detection reagents and all buffers required for colorimetric detection.

Features of the Cleaved Caspase 3 Colorimetric In-Cell ELISA Kit:

• Measures activated (cleaved) caspase 3 and tubulin in whole cells using 96-well or 384-well microplates
• colorimetric detection kit for use with standard microplate readers
• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative Western blotting
• Less costly (one well vs. one lane) than Western blotting

Related Products
Pierce™ Cleaved Caspase 3 Near Infrared In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
Detector Antibody Conjugate: Unconjugated (uses Secondary Antibody)
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: CPP32, CPP32B, SCA-1
Gene ID: 836
Gene Name: caspase 3
Gene Symbol: CASP3
Immunoassay Kit Format: In-Cell ELISA Kit
Immunoassay Kit Grade: Colorimetric
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Caspase-3
Target (Kit Named): Caspase 3
Target Species (Named): Multispecies
Target Species (Validated): Human
Target Sub-Specificity: Cleaved
UniProt ID: P42574

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 50 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• HRP Conjugate, 0.14 mL
• TMB Substrate, 58 mL
• TMB Stop Solution, 55 mL
• Janus Green Whole-Cell Stain, 50 mL
• Elution Buffer, 2 x 30 mL
• Thin Plate Seal Assembly, 8 each
• Anti-Cleaved Caspase 3 Antibody
• Anti-alpha Tubulin Antibody

Store antibodies as indicated on label. All other kit components should be stored at 4°C.

Documents

Manuals & protocols