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MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright™ 702, eBioscience™
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FIGURE: 1 / 35
MHC Class II (I-A/I-E) Antibody (67-5321-82) in Flow
Staining of mouse splenocytes with CD19 Monoclonal Antibody, FITC (Product # 12-0193-82) and 0.03 µg of Rat IgG2b K Isotype Control, Super Bright 702 (Product # 67-4031-82) (left) or 0.03 µg of MHC Class II (I-A/I-E) Monoclonal Antibody, Super Bright 702 (right). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
67-5321-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
M5/114.15.2
Conjugate
Super Bright™ 702
Super Bright™ 702
Super Bright™ 702
Excitation/Emission Max
413/702 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis.
Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: cell surface glycoprotein; class II Ia antigen A-beta; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H2-I-E-beta; H2-IA-beta cell surface glycoprotein; histocompatibility 2, class II antigen A, alpha, promoter 1; histocompatibility 2, class II antigen A, beta, promoter 1; histocompatibility 2, class II antigen E beta, promoter 1; I-Ab; I-Ad; I-Aq; I-Ar MHC class II molecule alpha chain; I-Ar MHC class II molecule beta chain; I-E beta MHC class II; I-Ed; I-Ek; IA-alpha polyprotein; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility protein class II alpha chain; MHC class 2; MHC class II antigen alpha chain; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC E-beta-b precursor; MHC H2-I-A-beta chain; MHC H2-IA-alpha; MHC H2-IE-beta; MHC H2-IE-beta cell surface glycoprotein; MHC NOD beta chain precursor; MHC-II I-A(d) alpha; MHC-II I-A(d) beta; response to metastatic cancers 1; unnamed protein product
Gene Aliases: Aalpha; Abeta; Eb; H-2Aa; H-2Ab; H-2Eb; H2-Ab; H2Aa; H2Eb; I-Aalpha; I-Abeta; Ia-1; Ia-2; Ia-4; Ia1; Ia2; Ia4; IAalpha; IAb; Rmcs1
UniProt ID: (Mouse) P14434, (Mouse) P14483
Entrez Gene ID: (Mouse) 14960, (Mouse) 14961, (Mouse) 14969
immune system process
peptide antigen assembly with MHC class II protein complex
immune response
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of immune response
positive regulation of T cell activation
negative regulation of T cell proliferation
positive regulation of T cell differentiation
antigen processing and presentation of peptide antigen
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