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Invitrogen
Phospho-PLCG1 (Tyr783) Recombinant Rabbit Monoclonal Antibody (C-p1p2-39H16L83)
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FIGURE: 1 / 3
Phospho-PLCG1 (Tyr783) Antibody (700044) in ICC/IF
Immunofluorescence analysis of Phospho-PLCG1 (Tyr783) was performed using 70% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-PLCG1 (Tyr783) Antibody (C-p1p2-39H16L83), Reco... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
700044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
C-p1p2-39H16L83
Immunogen
A peptide corresponding to amino acids 777-789 of P19174.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with bovine, mouse, rat, Xenopus and zebrafish based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; cell differentiation and embryonic development; EC 3.1.4.11; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; OTTHUMP00000178982; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-1; phospholipase C-148; phospholipase C-gamma; phospholipase C-II; Phospholipase C2; PLC gamma1; PLC-148; PLC-gamma-1; PLC2I; similar to PLC gamma 1; triphosphoinositide phosphodiesterase; unnamed protein product
Gene Aliases: Cded; IDAA; NCKAP3; Plc-1; Plc-gamma1; PLC-II; PLC1; PLC148; Plcg-1; PLCgamma1
UniProt ID: (Human) P19174, (Mouse) Q62077
Entrez Gene ID: (Human) 5335, (Mouse) 18803
phosphatidylinositol phospholipase C activity
phospholipase C activity
guanyl-nucleotide exchange factor activity
neurotrophin TRKA receptor binding
calcium ion binding
protein binding
phosphoric diester hydrolase activity
protein kinase binding
phosphatidylinositol phospholipase C activity
lysophospholipase C activity
insulin receptor binding
receptor tyrosine kinase binding
glutamate receptor binding
phosphoprotein binding
negative regulation of inflammatory response to antigenic stimulus
lipid metabolic process
signal transduction
epidermal growth factor receptor signaling pathway
phospholipid catabolic process
positive regulation of epithelial cell migration
cell migration
calcium-mediated signaling
intracellular signal transduction
Fc-epsilon receptor signaling pathway
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
phosphatidylinositol metabolic process
phosphatidylinositol-mediated signaling
antigen receptor-mediated signaling pathway
T cell receptor signaling pathway
release of sequestered calcium ion into cytosol
positive regulation of release of sequestered calcium ion into cytosol
cellular response to epidermal growth factor stimulus
positive regulation of vascular endothelial cell proliferation
positive regulation of endothelial cell apoptotic process
in utero embryonic development
calcium ion transport
positive regulation of cell migration
phosphatidylinositol catabolic process
inositol trisphosphate metabolic process
inositol trisphosphate biosynthetic process
positive regulation of cell size
modulation of synaptic transmission
positive regulation of wound healing
regulation of store-operated calcium channel activity
response to curcumin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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