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Invitrogen
Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Recombinant Rabbit Monoclonal Antibody (5H27L59)
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FIGURE: 1 / 5
Phospho-c-Met (Tyr1230, Tyr1234, Tyr1235) Antibody (700139) in ICC/IF
Immunofluorescence analysis of Phospho-Met pTyr1230+1234+1235 was done on 70% confluent log phase MKN45 cell treated with 10 ng of HGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Met pTyr1230+1234+1235 (5H27L59), Recombinant Rabbi... View More
Product Details
700139
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
5H27L59
Immunogen
proprietary
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with bovine, feline, mouse and rat based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met oncogene; MET proto-oncogene protein; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; tyrosine kinase; hepatocyte growth factor receptor; HGFR; Tyrosine-protein kinase Met; unnamed protein product
Gene Aliases: AUTS9; c-Met; DA11; DFNB97; HGFR; MET; RCCP2
UniProt ID: (Human) P08581
Entrez Gene ID: (Human) 4233
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
hepatocyte growth factor-activated receptor activity
protein binding
ATP binding
kinase activity
transferase activity
semaphorin receptor activity
protein phosphatase binding
identical protein binding
molecular function activator activity
endothelial cell morphogenesis
liver development
signal transduction
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of gene expression
negative regulation of autophagy
neuron differentiation
pancreas development
positive regulation of microtubule polymerization
negative regulation of Rho protein signal transduction
positive regulation of transcription from RNA polymerase II promoter
hepatocyte growth factor receptor signaling pathway
cell development
animal organ development
branching morphogenesis of an epithelial tube
positive chemotaxis
negative regulation of stress fiber assembly
excitatory postsynaptic potential
establishment of skin barrier
negative regulation of thrombin receptor signaling pathway
semaphorin-plexin signaling pathway
negative regulation of hydrogen peroxide-mediated programmed cell death
negative regulation of guanyl-nucleotide exchange factor activity
positive regulation of endothelial cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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