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Caspase 3 Recombinant Rabbit Monoclonal Antibody (9H19L2)
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FIGURE: 1 / 20
Caspase 3 Antibody (700182) in ICC/IF
Immunofluorescence analysis of Caspase-3 was done on 70% confluent log phase HeLa cells treated with 5 uM of Staurosporine for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Caspase-3 (9H19L2) Recombinant Rabbit Monoclonal Antibody (Product # 7001... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
700182
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
9H19L2
Immunogen
A peptide corresponding to amino acids 171-175 of P42574.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, feline, hamster, mouse, primate, pufferfish, rabbit, rat, porcine and Xenopus based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: apopain; Apopain precursor; CASP-3; caspase 3, apoptosis related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase3; CPP-32; cysteine protease CPP32; ICE3; PARP cleavage protease; procaspase3; protein Yama; SREBP cleavage activity 1; unnamed protein product
Gene Aliases: A830040C14Rik; AC-3; CASP-3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama
UniProt ID: (Human) P42574, (Mouse) P70677
Entrez Gene ID: (Human) 836, (Mouse) 12367
protease binding
endopeptidase activity
aspartic-type endopeptidase activity
cysteine-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
death receptor binding
protein binding
enzyme activator activity
peptidase activity
cysteine-type peptidase activity
phospholipase A2 activator activity
macromolecular complex binding
luteolysis
response to hypoxia
negative regulation of cytokine production
proteolysis
apoptotic process
axonal fasciculation
learning or memory
intrinsic apoptotic signaling pathway in response to osmotic stress
response to xenobiotic stimulus
response to glucose
response to metal ion
response to X-ray
regulation of macroautophagy
protein processing
hippocampus development
protein catabolic process
neuron differentiation
keratinocyte differentiation
erythrocyte differentiation
platelet formation
regulation of protein stability
response to cobalt ion
response to estradiol
response to lipopolysaccharide
regulation of protein localization
glial cell apoptotic process
response to tumor necrosis factor
response to nicotine
intracellular signal transduction
swimming behavior
response to hydrogen peroxide
positive regulation of apoptotic process
response to amino acid
regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
response to ethanol
neurotrophin TRK receptor signaling pathway
striated muscle cell differentiation
response to glucocorticoid
protein maturation
pyroptosis
leukocyte apoptotic process
response to anesthetic
cellular response to staurosporine
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
regulation of synaptic vesicle cycle
synapse pruning
positive regulation of pyroptotic inflammatory response
positive regulation of beta-amyloid formation
positive regulation of apoptotic DNA fragmentation
response to insulin-like growth factor stimulus
B cell homeostasis
cellular response to DNA damage stimulus
heart development
sensory perception of sound
response to UV
response to wounding
negative regulation of B cell proliferation
interleukin-18-mediated signaling pathway
T cell homeostasis
negative regulation of apoptotic process
cell fate commitment
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of cell cycle
negative regulation of activated T cell proliferation
neuron apoptotic process
anterior neural tube closure
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
protein poly-ADP-ribosylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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