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Invitrogen
Afadin Recombinant Rabbit Monoclonal Antibody (A7L9H48)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
700193
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse,
Rodent
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
A7L9H48
Immunogen
A peptide corresponding to amino acids 1805-1820 of Q9QZQ1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with human, rat, chimpanzee, Rhesus monkey, canine, bovine and chicken based on sequence homology. Boiling samples prior to Western blot is not recommended.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
AFDN belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: F-actin binding protein; FLJ34371; myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; protein Af-6; RP3-431P23.3; RP3-470B24.4; unnamed protein product
Gene Aliases: 5033403D15Rik; Af-6; AF6; Afadin; Gm314; I-afadin; Mllt4; S-afadin
UniProt ID: (Mouse) Q9QZQ1
Entrez Gene ID: (Mouse) 17356
signal transduction
positive regulation of gene expression
telencephalon development
cerebral cortex development
positive regulation of cell-cell adhesion
cell differentiation
negative regulation of cell migration
cell junction organization
adherens junction maintenance
cell-cell adhesion mediated by cadherin
pore complex assembly
brain morphogenesis
homeostasis of number of cells
positive regulation of dendrite morphogenesis
radial glial cell differentiation
neuroepithelial cell differentiation
positive regulation of dendritic spine morphogenesis
positive regulation of mini excitatory postsynaptic potential
establishment of protein localization to plasma membrane
regulation of oligodendrocyte progenitor proliferation
bicellular tight junction assembly
establishment of endothelial intestinal barrier
postsynaptic specialization organization
regulation of postsynapse organization
dendrite arborization
regulation of postsynapse assembly
protein localization to cell junction
positive regulation of dendrite extension
positive regulation of cell-cell adhesion mediated by cadherin
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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