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FIGURE: 1 / 14
LC3B Antibody (700712) in ICC/IF
Immunofluorescent analysis of LC3B was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with LC3B Recombinant Rabbit Monoclonal Antibody (Product # 700712) at 2 µg/mL and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at r... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
700712
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H30L32
Immunogen
Recombinant human LC3B protein corresponding to amino acids 2-120
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATG8E; autophagy-related protein LC3 B; autophagy-related ubiquitin-like modifier LC3 B; LC3 beta; LC3A; lc3b; lc3b3; MAP1 light chain 3-like protein 2; MAP1A/1B; MAP1A/1B LC3; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B LC3 B-like; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B light chain 3 B-like; MAP1A1B-LC3; MAP1BLC3; MAP1LC3A; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like protein; RP11-346K17.1; unnamed protein product
Gene Aliases: 1010001C15Rik; Atg8; ATG8F; ATG8G; LC3B; MAP1A/1BLC3; MAP1A/MAP1B; Map1lc3; MAP1LC3B-a; Mpl3; zbs559
UniProt ID: (Human) Q9GZQ8, (Human) A6NCE7, (Mouse) Q9CQV6, (Rat) Q62625
Entrez Gene ID: (Human) 81631, (Human) 643246, (Mouse) 67443, (Rat) 64862
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