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          • Primary Antibodies ›
          • Digoxigenin Antibodies

          Invitrogen

          Digoxigenin Recombinant Rabbit Monoclonal Antibody (9H27L19)

          1 Published Figure
          19 References
          View all (8) Digoxigenin antibodies

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          Cite Digoxigenin Recombinant Rabbit Monoclonal Antibody (9H27L19)

          Additional Information:

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          • Antibody Testing Data (1)
          • Published Figures (1)
          Digoxigenin Antibody in Western Blot (WB)
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          Digoxigenin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Digoxigenin Antibody (700772) in WB

          Western blot analysis of recombinant Digoxigenin conjugated to BSA (1 ng/lane) using a Digoxigenin recombinant rabbit monoclonal antibody (Product # 700772) at a dilution of 0.1 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Digoxigenin Antibody in Western Blot (WB)
          Digoxigenin Antibody in Immunocytochemistry (ICC/IF)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          700772

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunocytochemistry (ICC/IF)

          -
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 3 publications 3 publications

          Functional Assay (Functional)

          -
          View 1 publication 1 publication

          In Situ Hybridization (ISH)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (Misc)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Bacteria, Chemical, Fungi, Mouse, Rat, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          9H27L19

          Immunogen

          proprietary

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.2-7.4

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2532342

          Product Specific Information

          Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          Digoxin, also known as digitalis, is a purified cardiac glycoside extracted from the foxglove plant, Digitalis lanata. Its corresponding aglycone is digoxigenin, and its acetyl derivative is acetyldigoxin. Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and sometimes heart failure that cannot be controlled by other medication. Digoxin preparations are commonly marketed under the trade names Lanoxin, Digitek, and Lanoxicaps. Digoxin is also used as an affinity system for nonradioactive labeling and detection of proteins and nucleic acids.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: C23H34O5; DIG; Digitalis

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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