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Invitrogen
JNK1 Recombinant Rabbit Monoclonal Antibody (2H15L8)
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FIGURE: 1 / 2
JNK1 Antibody (701048) in ICC/IF
Immunofluorescence analysis of JNK1 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JNK1/MAPK8 (2H15L8) Recombinant Rabbit Monoclonal Antibody (Product # 701048) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugat... View More
Product Details
701048
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H15L8
Immunogen
Recombinant protein corresponding to amino acids 17-126 of human c-Jun N-terminal kinase 1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
MAPK8 (JNK1) is activated by the presence of misfolded proteins in the endoplasmic reticulum. It is also a crucial mediator of insulin resistance and obesity. It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Jun N-terminal kinase 1; FLJ12099; FLJ33785; JNK-46; JNK3; JNK3A; JUN N-terminal kinase; MAP kinase 10; MAP kinase 8; MAPK 10; MAPK10; MAPK8; MGC50974; Mitogen-activated protein kinase 8; MK10; OTTHUMP00000019552; p493F12; p54bSAPK; Phospho-JNK; PRKM10; protein kinase JNK1; SAPK1c; stress-activated protein kinase 1; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; unnamed protein product
Gene Aliases: JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; MAPK8; PRKM8; SAPK1; SAPK1C
UniProt ID: (Human) P45983
Entrez Gene ID: (Human) 5599
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
JUN kinase activity
MAP kinase activity
protein binding
ATP binding
kinase activity
transferase activity
enzyme binding
protein phosphatase binding
histone deacetylase regulator activity
histone deacetylase binding
protein serine kinase activity
protein serine/threonine kinase binding
MAPK cascade
double-strand break repair
protein phosphorylation
response to oxidative stress
JNK cascade
JUN phosphorylation
response to UV
response to mechanical stimulus
positive regulation of gene expression
regulation of macroautophagy
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
positive regulation of cyclase activity
positive regulation of cell killing
negative regulation of protein binding
regulation of protein localization
cellular response to amino acid starvation
cellular response to oxidative stress
cellular response to reactive oxygen species
Fc-epsilon receptor signaling pathway
regulation of circadian rhythm
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of mRNA stability
NLRP3 inflammasome complex assembly
rhythmic process
positive regulation of protein metabolic process
stress-activated MAPK cascade
mRNA destabilization
protein poly-ADP-ribosylation
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular senescence
energy homeostasis
positive regulation of NLRP3 inflammasome complex assembly
positive regulation of establishment of protein localization to mitochondrion
protein localization to site of double-strand break
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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