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Invitrogen
PITX3 Recombinant Rabbit Monoclonal Antibody (5H10L5)
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FIGURE: 1 / 1
PITX3 Antibody (701181) in ICC/IF
Immunofluorescent analysis of PITX3 in U2OS cells using a PITX3 recombinant rabbit monoclonal antibody (Product # 701181) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B). c: Membrane-bound glycoconjugates on the cell surface stained with Alexa Fluor 594 wheat germ agglutinin (red). Image D is a composite image showing nuclear localization of PiTX3.
Product Details
701181
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
5H10L5
Immunogen
Recombinant protein corresponding to amino acids 1-65 of human PiTX3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Homeobox protein PITX3; MGC12766; Paired-like homeodomain transcription factor 3; Pituitary homeobox 3
Gene Aliases: ASGD1; ASMD; ASOD; CTPP4; CTRCT11; PITX3; PTX3
UniProt ID: (Human) O75364
Entrez Gene ID: (Human) 5309
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific double-stranded DNA binding
lens development in camera-type eye
lens morphogenesis in camera-type eye
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
locomotory behavior
anatomical structure morphogenesis
organ morphogenesis
regulation of gene expression
negative regulation of gliogenesis
midbrain development
response to immobilization stress
response to cocaine
positive regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
neuron development
negative regulation of neurogenesis
lens fiber cell differentiation
dopaminergic neuron differentiation
response to methamphetamine hydrochloride
positive regulation of cell proliferation in midbrain
cellular response to glial cell derived neurotrophic factor
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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