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Invitrogen

mTOR Recombinant Rabbit Monoclonal Antibody (7H13L18)

2 Published Figures
3 References
View all (66) mTOR antibodies

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mTOR Recombinant Rabbit Monoclonal Antibody (7H13L18)

Product Details

701483

Applications
Tested Dilution
Publications

Western Blot (WB)

2-3 µg/mL
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

7H13L18

Immunogen

Recombinant protein corresponding to amino acids 1126-1411 of human mTOR
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532486

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTORC1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; tyrosine-protein kinase mTOR; unnamed protein product

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Gene Aliases: FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS

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UniProt ID: (Human) P42345

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Entrez Gene ID: (Human) 2475

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Function(s)
inositol hexakisphosphate binding RNA polymerase III type 1 promoter sequence-specific DNA binding RNA polymerase III type 2 promoter sequence-specific DNA binding RNA polymerase III type 3 promoter sequence-specific DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding kinase activity identical protein binding ribosome binding ion channel binding macromolecular complex binding phosphoprotein binding protein serine kinase activity
Process(es)
regulation of cell growth T-helper 1 cell lineage commitment 'de novo' pyrimidine nucleobase biosynthetic process protein phosphorylation inflammatory response cellular response to DNA damage stimulus cytoskeleton organization regulation of cell size cellular response to starvation response to heat regulation of autophagy negative regulation of autophagy positive regulation of epithelial to mesenchymal transition regulation of macroautophagy negative regulation of macroautophagy regulation of lipid metabolic process neuronal action potential positive regulation of cell growth T cell costimulation response to nutrient levels cellular response to nutrient levels cellular response to nutrient TOR signaling cellular response to insulin stimulus regulation of actin cytoskeleton organization cellular response to amino acid starvation TORC1 signaling TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process response to amino acid anoikis protein kinase B signaling positive regulation of cell differentiation regulation of osteoclast differentiation positive regulation of translation positive regulation of glycolytic process positive regulation of protein kinase activity positive regulation of transcription from RNA polymerase III promoter positive regulation of translational initiation negative regulation of insulin receptor signaling pathway positive regulation of lipid biosynthetic process behavioral response to pain protein stabilization positive regulation of multicellular organismal process positive regulation of keratinocyte migration regulation of protein kinase B signaling positive regulation of protein kinase B signaling cardiac cell development cellular response to methionine positive regulation of SCF-dependent proteasomal ubiquitin-dependent catabolic process cellular response to amino acid stimulus cellular response to leucine cellular response to hypoxia cellular response to osmotic stress regulation of cellular response to stress vascular endothelial cell response to laminar fluid shear stress regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of wound healing, spreading of epidermal cells regulation of locomotor rhythm positive regulation of cytoplasmic translational initiation regulation of lysosome organization negative regulation of lysosome organization positive regulation of pentose-phosphate shunt cellular response to leucine starvation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of autophagosome assembly
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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