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FIGURE: 1 / 10
Optineurin Antibody (702766) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous Optineurin using Anti- Optineurin Recombinant Rabbit Monoclonal Antibody (Product # 702766, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2,000). Panel a) shows representative cells that were stained for de... View More
Product Details
702766
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
22H12L20
Immunogen
Peptides corresponding to human Optineurin [1) aa171-aa186, 2) aa27-aa42]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Horse, cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14. 7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14.7K-interacting protein 2; Ag9 C5; E3-14.7K-interacting protein; FIP 2; FIP-2; FIP-2-like protein; FIP-2; HYPL; NRP; TFIIIA-INTP; RAB8-INTP; E3-14.7K-INTP; HIP 7; HIP-7; Huntingtin interacting protein L; Huntingtin yeast partner L; Huntingtin-interacting protein 7; Huntingtin-interacting protein L; interacts with adenovirus E3-14.7KDa, a TNF-alpha cytolysis antagonist; NEMO-related protein; Optic neuropathy-inducing protein; Optineurin; TFIIIA IntP; TFIIIA-INTP; Transcription factor IIIA-interacting protein; transcrption factor IIIA-interacting protein; tumor necrosis factor alpha-inducible cellular protein containing leucine zipper domains; Uban; unnamed protein product
Gene Aliases: 4930441O07Rik; ALS12; FIP2; GLC1E; HIP7; HYPL; NRP; OPTN; TFIIIA-INTP
UniProt ID: (Human) Q96CV9, (Rat) Q8R5M4, (Mouse) Q8K3K8
Entrez Gene ID: (Human) 10133, (Rat) 246294, (Mouse) 71648
protein targeting to Golgi
negative regulation of receptor recycling
autophagy
Golgi organization
positive regulation of cell proliferation
macroautophagy
cellular protein localization
wound healing
Golgi to plasma membrane protein transport
regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of neuron apoptotic process
cytokine secretion
defense response to Gram-negative bacterium
protein homooligomerization
Golgi ribbon formation
immune system process
signal transduction
protein localization
cell death
positive regulation of autophagy
protein localization to Golgi apparatus
cellular response to unfolded protein
innate immune response
parkin-mediated mitophagy in response to mitochondrial depolarization
positive regulation of xenophagy
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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