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Invitrogen
SAV1 Recombinant Rabbit Monoclonal Antibody (6H5L16)
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FIGURE: 1 / 3
SAV1 Antibody (703002) in ICC/IF
Knockdown of SAV1 was achieved by transfecting HCT 116 cells with specific siRNA (Silencer® select Product # s34083, s34081). Immunofluorescence analysis was performed on HCT 116 cells (untransfected, panel a-d), transfected with SAV1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-SAV1 Recombinant Rabbit Monoclonal Antibody (Product # 703002, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More
Product Details
703002
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H5L16
Immunogen
Protein corresponding to human SAV1 [aa300-aa383]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicting to react with Monkey, Cat, Dog.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Sav1 is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. It also plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, Sav1 activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1700040G09Rik; 45 kDa WW domain protein; hWW45; Protein salvador homolog 1; salvador homolog 1; SAV1; unnamed protein product; WW domain-containing adaptor 45
Gene Aliases: SAV; SAV1; WW45; WWP4
UniProt ID: (Human) Q9H4B6
Entrez Gene ID: (Human) 60485
hair follicle development
apoptotic process
signal transduction
negative regulation of cell proliferation
regulation of gene expression
keratinocyte differentiation
hippo signaling
negative regulation of hippo signaling
positive regulation of hippo signaling
regulation of cell proliferation
positive regulation of apoptotic process
positive regulation of fat cell differentiation
regulation of organ growth
epithelial cell proliferation
negative regulation of epithelial cell proliferation
protein stabilization
cardiac muscle cell proliferation
negative regulation of cardiac muscle cell proliferation
ventricular septum morphogenesis
lung epithelial cell differentiation
intestinal epithelial cell differentiation
keratinocyte apoptotic process
positive regulation of keratinocyte apoptotic process
regulation of stem cell population maintenance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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