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Invitrogen

NFkB p65 Recombinant Superclonal™ Antibody (4-2HCLC)

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4 Published Figures
4 References
View all (142) NFkB p65 antibodies

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NFkB p65 Recombinant Superclonal™ Antibody (4-2HCLC)

Product Details

710048

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1 µg/mL
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
-

Immunocytochemistry (ICC/IF)

1 µg/mL
View 2 publications 2 publications

ELISA (ELISA)

Assay-Dependent
-

ChIP assay (ChIP)

3 µg
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

4-2HCLC

Immunogen

Recombinant protein corresponding to amino acids 375-551 of human NF kappaB p65.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532539

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; EBP-1; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; unnamed protein product; v-rel avian reticuloendotheliosis viral oncogene homolog A

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Gene Aliases: AIF3BL3; CMCU; NFKB3; p65; RELA

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UniProt ID: (Human) Q04206, (Mouse) Q04207

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Entrez Gene ID: (Human) 5970, (Mouse) 19697

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding enzyme binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding peptide binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding sequence-specific DNA binding macromolecular complex binding NF-kappaB binding ankyrin repeat binding general transcription initiation factor binding DNA-binding transcription factor binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor binding protein complex binding activating transcription factor binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding repressing transcription factor binding Rel homology transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter negative regulation of cytokine production liver development hair follicle development defense response to tumor cell response to ischemia chromatin organization transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response inflammatory response cellular defense response neuropeptide signaling pathway I-kappaB kinase/NF-kappaB signaling positive regulation of cell proliferation response to xenobiotic stimulus response to mechanical stimulus response to bacterium organ morphogenesis response to UV-B positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of Schwann cell differentiation negative regulation of angiogenesis cytokine-mediated signaling pathway signal transduction involved in regulation of gene expression protein catabolic process response to muramyl dipeptide response to lipopolysaccharide response to progesterone positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production response to insulin tumor necrosis factor-mediated signaling pathway negative regulation of protein sumoylation response to cobalamin response to cytokine toll-like receptor 4 signaling pathway intracellular signal transduction cellular response to hepatocyte growth factor stimulus response to muscle stretch NIK/NF-kappaB signaling vascular endothelial growth factor signaling pathway toll-like receptor TLR6:TLR2 signaling pathway prolactin signaling pathway negative regulation of protein catabolic process response to hydrogen peroxide negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid response to morphine innate immune response response to ethanol negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin receptor signaling pathway regulation of inflammatory response positive regulation of T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity response to cAMP defense response to virus cellular response to hydrogen peroxide nucleotide-binding oligomerization domain containing 2 signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to peptidoglycan cellular response to nicotine cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor postsynapse to nucleus signaling pathway antiviral innate immune response negative regulation of cytokine production involved in inflammatory response negative regulation of NIK/NF-kappaB signaling positive regulation of NIK/NF-kappaB signaling positive regulation of beta-amyloid formation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter cellular response to angiotensin positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of miRNA metabolic process negative regulation of extrinsic apoptotic signaling pathway transcription from RNA polymerase II promoter response to organic substance positive regulation of chondrocyte differentiation positive regulation of interleukin-12 biosynthetic process cellular response to peptide hormone stimulus
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