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ANGPTL4 Antibody (710186) in ICC/IF
Immunofluorescence analysis of ANGPTL4 was performed using 70% confluent log phase HUVEC cells treated with 150uM of Cobalt Chloride for 24 h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ANGPTL4 (1HCLC) Recombinant Rabbit Superclonal™ Antibody (Product # 710186) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
710186
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
1HCLC
Immunogen
Peptide corresponding to amino acids 258-273 of human Angiopoietin-like 4
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Angiopoietin-like protein 4 (ANGPTL4), is a secreted protein selectively expressed in adipose tissue, liver, and placenta1-3 that plays a variety of roles in vivo, ranging from adipogenesis to angiogenesis to carcinogenesis. The expression of ANGPTL4 is also under nutritional and hormonal control. ANGPTL4 has been observed to induce a strong pro-angiogenic response independent of vascular endothelial growth factor (VEGF), and its expression has been described in hypoxic human tissues as well as a variety of cancers, including liposarcoma, hepatocellular carcinoma, and conventional renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 425O18-1; angiopoietin-like protein 4; ANGPTL2; contains fibrinogen-like domain; Fasting-i; fasting-induced adipose factor; fibrinogen/angiopoietin-related protein; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; major histocompatibility complex region NG27; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein; PPARG angiopoietin related protein; secreted protein; secreted protein Bk89; unnamed protein product
Gene Aliases: ARP4; Bk89; FIAF; HARP; HFARP; Ng27; NL2; PGAR; Pgarg; Pp1158; TGQTL; UNQ171
UniProt ID: (Human) Q9BY76, (Mouse) Q9Z1P8
Entrez Gene ID: (Human) 51129, (Mouse) 57875
response to hypoxia
blood coagulation
negative regulation of very-low-density lipoprotein particle remodeling
negative regulation of apoptotic process
protein unfolding
negative regulation of fatty acid biosynthetic process
positive regulation of angiogenesis
positive regulation of multicellular organismal process
triglyceride homeostasis
regulation of chylomicron remodeling
regulation of triglyceride transport
cellular response to starvation
positive regulation of lipid metabolic process
negative regulation of lipoprotein lipase activity
negative regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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