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FIGURE: 1 / 3
beta-2 Microglobulin Antibody (710274) in WB
Western blot analysis of B2M in whole cell extracts from HeLa (lane 1), U937 (lane 2), and A431 cells (lane 3) using a B2M Recombinant Rabbit Superclonal™ Antibody (Product # 710274) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~12kDa.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
710274
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
4HCLC
Immunogen
Recombinant protein corresponding to amino acids 21-119 of human b2-microglobulin.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-F; B2-microglobulin; B2M; Beta 2 microglobin; Beta 2 microglobulin; beta 2-microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta2 microglobulin; HDCMA22P; MHC Class I; microglobulin beta-2; Orla-B2m; similar to Homo sapiens mRNA for beta 2-microglobulin with GenBank Accession Number AB021288; unnamed protein product
Gene Aliases: AMYLD6; IMD43; MHC1D4
UniProt ID: (Human) P61769
Entrez Gene ID: (Human) 567
antigen processing and presentation of exogenous peptide antigen via MHC class Ib
peptide antigen assembly with MHC class I protein complex
peptide antigen assembly with MHC class II protein complex
regulation of natural killer cell mediated immunity
positive regulation of T cell cytokine production
regulation of membrane depolarization
cellular iron ion homeostasis
immune response
sensory perception of smell
learning or memory
response to metal ion
antibacterial humoral response
antigen processing and presentation of endogenous peptide antigen via MHC class I
antigen processing and presentation of exogenous peptide antigen via MHC class II
transferrin transport
regulation of iron ion transport
negative regulation of iron ion transport
innate immune response
positive regulation of receptor-mediated endocytosis
negative regulation of receptor-mediated endocytosis
negative regulation of epithelial cell proliferation
negative regulation of neurogenesis
positive regulation of immune response
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of T cell activation
protein homotetramerization
antimicrobial humoral immune response mediated by antimicrobial peptide
cellular response to lipopolysaccharide
cellular response to iron ion
cellular response to nicotine
amyloid fibril formation
positive regulation of cellular senescence
negative regulation of forebrain neuron differentiation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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