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Invitrogen

Phospho-Rb (Thr821) Recombinant Superclonal™ Antibody (16HCLC)

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Phospho-Rb (Thr821) Recombinant Superclonal™ Antibody (16HCLC)

Product Details

710314

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:20
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

16HCLC

Immunogen

Phosphopeptide corresponding to amino acids 817-825 of human Retinoblastoma [pT821]
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532682

Product Specific Information

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; p105-Rb; P105RB; p110-RB1; Phospho-Retinoblastoma; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma gene; retinoblastoma suceptibility protein; retinoblastoma susceptibility protein; retinoblastoma suspectibility protein; Retinoblastoma-associated protein; unnamed protein product

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Gene Aliases: OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; RB; RB1

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UniProt ID: (Human) P06400

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Entrez Gene ID: (Human) 5925

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding DNA binding transcription corepressor activity protein binding enzyme binding kinase binding ubiquitin protein ligase binding identical protein binding phosphoprotein binding binding, bridging RNA polymerase II sequence-specific DNA binding transcription factor binding importin-alpha family protein binding disordered domain specific binding DNA-binding transcription factor binding
Process(es)
G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter regulation of cell growth tissue homeostasis chondrocyte differentiation aortic valve morphogenesis chromatin organization chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter negative regulation of protein kinase activity apoptotic process smoothened signaling pathway Ras protein signal transduction spermatogenesis regulation of mitotic cell cycle cell proliferation negative regulation of cell proliferation negative regulation of gene expression glial cell proliferation cell differentiation neuron differentiation negative regulation of cell growth sister chromatid biorientation neuron projection development heterochromatin assembly developmental process cellular response to insulin stimulus maintenance of mitotic sister chromatid cohesion glial cell apoptotic process skeletal muscle cell differentiation neuron maturation enucleate erythrocyte differentiation regulation of lipid kinase activity myoblast differentiation positive regulation of macrophage differentiation negative regulation of cell cycle positive regulation of mitotic metaphase/anaphase transition negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter digestive tract development cell morphogenesis involved in neuron differentiation epithelial cell proliferation negative regulation of epithelial cell proliferation negative regulation of inflammatory response striated muscle cell differentiation chromosome organization cell division neuron apoptotic process regulation of cell cycle negative regulation of glial cell proliferation protein localization to chromosome, centromeric region cellular response to xenobiotic stimulus regulation of centromere complex assembly hepatocyte apoptotic process negative regulation of cold-induced thermogenesis positive regulation of extracellular matrix organization negative regulation of hepatocyte apoptotic process positive regulation of collagen fibril organization negative regulation of myofibroblast differentiation regulation of G1/S transition of mitotic cell cycle negative regulation of G1/S transition of mitotic cell cycle positive regulation of transcription regulatory region DNA binding negative regulation of apoptotic signaling pathway
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