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FIGURE: 1 / 3
CXCL10 Antibody (710399) in ICC/IF
Immunofluorescence analysis of C-X-C motif chemokine 10 was performed using 70% confluent log phase HT-29 cells and HT-29 cells treated with 50 ng/mL IFN gamma for 18h followed by 1x PTI treatment for 4h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CXCL10 Recombinant Rabbit Monoclonal Antibody (10HCLC) (Product # 710399) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
710399
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
10HCLC
Immunogen
proprietary
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with rat and porcine based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; CXC; CXC motif chemokine; CXCL; gamma IP10; gamma-IP10; H-IP-10; interferon activated gene 10; interferon-gamma induced protein CRG-2; interferon-inducible cytokine IP-10; M-IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10; unnamed protein product
Gene Aliases: C7; CRG-2; gIP-10; IFI10; INP10; IP-10; IP10; mob-1; SCYB10
UniProt ID: (Human) P02778, (Mouse) P17515
Entrez Gene ID: (Human) 3627, (Mouse) 15945
positive regulation of leukocyte chemotaxis
chemotaxis
defense response
inflammatory response
immune response
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
positive regulation of cell proliferation
response to bacterium
T cell chemotaxis
regulation of T cell chemotaxis
negative regulation of angiogenesis
positive regulation of cell migration
neutrophil chemotaxis
killing of cells of other organism
endothelial cell activation
regulation of cell proliferation
regulation of apoptotic process
negative regulation of myoblast differentiation
positive chemotaxis
positive regulation of release of sequestered calcium ion into cytosol
defense response to virus
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
positive regulation of monocyte chemotaxis
cellular response to interleukin-17
cellular response to virus
antiviral innate immune response
regulation of endothelial tube morphogenesis
negative regulation of myoblast fusion
positive regulation of T cell migration
cell surface receptor signaling pathway
cell-cell signaling
muscle organ development
blood circulation
response to gamma radiation
response to auditory stimulus
response to lipopolysaccharide
response to vitamin D
cellular response to heat
positive regulation of transcription from RNA polymerase II promoter
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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