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Connexin 43 Polyclonal Antibody
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FIGURE: 1 / 91
Connexin 43 Antibody (71-0700) in IHC (P)
Immunohistochemistry analysis of CONNEXIN 43 showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperatur... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
71-0700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
Cat,
Dog,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Tag,
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide corresponding to a segment of the 3rd cytoplasmic domain (C-terminal portion) of rat connexin 43.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with connexin 43. On western blots, this antibody detects a 43 kDa protein with mouse brain, rat brain, and human smooth muscle cell extracts. Reactivity with other connexin types has not been observed on western blots (note: samples were not boiled prior to running SDS-PAGE). Positive immunohistostaining is seen with mouse heart and thalamus tissue. Paraffin embedded sections require a heat-induced epitope retrieval (HIER) step. Reactivity of this antibody with connexin 43 is independent of phosphorylation status.
Target Information
Connexin 43 (Cx43), encoded by the GJA1 gene, is a widely expressed gap junction protein that plays an important role in direct cell-to-cell communication. Six Cx43 proteins assemble to form a connexon, or hemichannel, which can pair with a connexon on a neighboring cell to create a gap junction channel. These channels allow ions, metabolites, and small signaling molecules to pass between adjacent cells, helping coordinate processes such as electrical conduction, tissue development, wound healing, and cellular homeostasis. Cx43 is especially abundant in tissues such as the heart, brain, bone, and skin, and changes in its expression, localization, or function have been associated with cardiovascular disease, neurological disorders, impaired tissue repair, and cancer. Because of its broad role in intercellular signaling and disease biology, Cx43 is an important target for studying gap junction function and developing potential therapeutic strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha 1 connexin; connexin 43; connexin k1; connexin-43; Cx-43; CXA1; DFNB38; gap junction 43 kDa heart protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; GJA1; Npm1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product; VEGF
Gene Aliases: AVSD3; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxnk1; EKVP; EKVP3; Gja-1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA
UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050
Entrez Gene ID: (Human) 2697, (Mouse) 14609, (Rat) 24392
receptor binding
gap junction channel activity
protein binding
beta-catenin binding
ion transmembrane transporter activity
efflux transmembrane transporter activity
tubulin binding
transmembrane transporter activity
glutathione transmembrane transporter activity
alpha-tubulin binding
beta-tubulin binding
gap junction hemi-channel activity
gap junction channel activity involved in cardiac conduction electrical coupling
scaffold protein binding
gap junction channel activity involved in cell communication by electrical coupling
protein tyrosine kinase binding
channel activity
SH3 domain binding
protein domain specific binding
PDZ domain binding
connexin binding
disordered domain specific binding
establishment of mitotic spindle orientation
osteoblast differentiation
in utero embryonic development
neuron migration
negative regulation of endothelial cell proliferation
heart looping
epithelial cell maturation
lens development in camera-type eye
chronic inflammatory response
positive regulation of glomerular filtration
atrial ventricular junction remodeling
epicardial cell to mesenchymal cell transition
cell communication
signal transduction
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
spermatogenesis
heart development
adult heart development
regulation of heart contraction
protein localization
negative regulation of cell proliferation
male gonad development
response to pH
vascular transport
positive regulation of gene expression
negative regulation of gene expression
cell communication by chemical coupling
cell communication by electrical coupling
regulation of cell communication by electrical coupling
positive regulation of cell communication by chemical coupling
glutamate secretion
ATP transport
negative regulation of cell growth
regulation of bone mineralization
positive regulation of insulin secretion
negative regulation of gonadotropin secretion
ion transmembrane transport
glutathione transmembrane transport
embryonic heart tube development
maintenance of protein localization in endoplasmic reticulum
T cell proliferation
T cell activation
vasodilation
embryonic digit morphogenesis
xenobiotic transport
regulation of apoptotic process
skeletal muscle tissue regeneration
cell-cell junction organization
positive regulation of protein catabolic process
positive regulation of meiotic nuclear division
positive regulation of striated muscle tissue development
positive regulation of vasoconstriction
bone remodeling
regulation of bone remodeling
blood vessel morphogenesis
neuron projection morphogenesis
regulation of body fluid levels
regulation of calcium ion transport
transmembrane transport
negative regulation of cardiac muscle cell proliferation
milk ejection
regulation of ventricular cardiac muscle cell membrane repolarization
regulation of blood vessel remodeling
bone development
regulation of atrial cardiac muscle cell membrane depolarization
regulation of ventricular cardiac muscle cell membrane depolarization
cardiac conduction
cellular response to pH
cell communication by electrical coupling involved in cardiac conduction
establishment of epithelial cell polarity
microtubule-based transport
regulation of actin filament organization
positive regulation of cold-induced thermogenesis
export across plasma membrane
negative regulation of trophoblast cell migration
regulation of blood circulation
positive regulation of establishment of Sertoli cell barrier
cellular response to beta-amyloid
positive regulation of vascular smooth muscle cell proliferation
positive regulation of morphogenesis of an epithelium
positive regulation of mesodermal cell differentiation
negative regulation of DNA biosynthetic process
positive regulation of stem cell proliferation
regulation of bicellular tight junction assembly
positive regulation of behavioral fear response
response to ischemia
endothelium development
response to wounding
response to glucose
gap junction assembly
response to estradiol
response to lipopolysaccharide
response to retinoic acid
response to fluid shear stress
cellular response to platelet-derived growth factor stimulus
response to peptide hormone
decidualization
cellular response to mechanical stimulus
cellular response to parathyroid hormone stimulus
positive regulation of smooth muscle cell chemotaxis
epididymis development
cardiac conduction system development
maintenance of blood-brain barrier
positive regulation of I-kappaB kinase/NF-kappaB signaling
atrial cardiac muscle cell action potential
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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