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FIGURE: 1 / 4
Phospho-c-Met (Tyr1349) Antibody (710792) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized MKN45 cells which were serum starved for 16 hours and treated with 100 ng of HGF/10 min for detection of cMet (pY1349) using Anti-cMet pY1349 Recombinant Rabbit Superclonal™ Antibody (Product # 710792, 1-2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
710792
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
15HCLC
Immunogen
Phosphorylated peptide corresponding to Human MET (aa 1346-1352)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Pig, Bovine, Feline, Goat, Rat and Mouse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cMet; EC 2.7.10.1; HGF receptor; HGF-SF receptor; HGF/SF receptor; met oncogene; MET proto-oncogene protein; oncogene MET; proto-oncogene c-Met; sc MET; scatter factor receptor; SF receptor; soluble c met; tyrosine kinase; hepatocyte growth factor receptor; HGFR; tyrosine-protein kinase Met; unnamed protein product
Gene Aliases: AUTS9; c-Met; DA11; DFNB97; HGFR; RCCP2
UniProt ID: (Human) P08581
Entrez Gene ID: (Human) 4233
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
hepatocyte growth factor-activated receptor activity
protein binding
ATP binding
semaphorin receptor activity
protein phosphatase binding
identical protein binding
molecular function activator activity
endothelial cell morphogenesis
liver development
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of gene expression
negative regulation of autophagy
neuron differentiation
pancreas development
positive regulation of transcription from RNA polymerase II promoter
hepatocyte growth factor receptor signaling pathway
cell development
branching morphogenesis of an epithelial tube
positive chemotaxis
excitatory postsynaptic potential
semaphorin-plexin signaling pathway
negative regulation of hydrogen peroxide-mediated programmed cell death
positive regulation of endothelial cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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