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FIGURE: 1 / 3
Phospho-c-Jun (Thr91, Thr93, Thr95) Antibody (711207) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of COLO 205 (Lane 1), U-937 (Lane 2), PC-3 (Lane 3) and U-87 MG (Lane 4). The blots were probed with Anti-c-jun (pT91/93/95) Recombinant Rabbit Superclonal™ Antibody (Product # 711207, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036,... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
711207
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
13HCLC
Immunogen
Peptide corresponding to Human JUN (aa 86-96)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig and Mouse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activator protein 1; cjun; enhancer-binding protein AP1; JUN; Jun activation domain binding protein; jun oncogene; JUND; proto-oncogene c-Jun; proto-oncogene cJun; transcription factor AP-1; transcription factor AP-1 subunit Jun; v-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
Gene Aliases: AP-1; AP1; c-Jun; cJUN; p39
UniProt ID: (Human) P05412
Entrez Gene ID: (Human) 3725
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
GTPase activator activity
protein binding
enzyme binding
ubiquitin protein ligase binding
cAMP response element binding
identical protein binding
ubiquitin-like protein ligase binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
R-SMAD binding
general transcription initiation factor binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
JNK cascade
release from viral latency
transforming growth factor beta1 production
response to endoplasmic reticulum stress
regulation of cell proliferation
negative regulation of DNA binding
negative regulation by host of viral transcription
positive regulation by host of viral transcription
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to steroid hormone
regulation of cell cycle
SMAD protein signal transduction
cellular response to anisomycin
integrated stress response signaling
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of vascular smooth muscle cell proliferation
positive regulation of DNA-templated transcription, initiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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