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          • Primary Antibodies ›
          • GSK3 alpha/beta Antibodies
          Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Invitrogen

          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Superclonal™ Antibody

          View all (24) GSK3 alpha/beta antibodies

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          Cite Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Superclonal™ Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody (711262) in ICC/IF

          For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous GSK-3 alpha (pY279) + GSK3 beta (pY216) using Anti-GSK-3 alpha (pY279) + GSK3 beta (pY216) Recombinant Rabbit Superclonal™ Antibody (Product # 711262, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Superclonal™ Antibody

          Product Details

          711262

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Superclonal

          Type

          Antibody

          Immunogen

          Peptide corresponding to Human GSK3A, GSK3B (aa 211-220)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.2-7.4

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2632972

          Product Specific Information

          This antibody is predicted to react with Monkey, rabbit and horse.

          Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Target Information

          Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: GSK-3 alpha; GSK-3 beta; gsk-3-alpha; GSK3B; kinase; mGSK3beta; serine/threonine-protein kinase GSK3A; serine/threonine-protein kinase GSK3B; unnamed protein product

          View more View less

          Gene Aliases: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; GSK-3; GSK-3beta; GSK3

          View more View less

          UniProt ID: (Human) P49840, (Human) P49841, (Mouse) Q2NL51, (Mouse) Q9WV60

          View more View less

          Entrez Gene ID: (Human) 2931, (Human) 2932, (Mouse) 606496, (Mouse) 56637

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity receptor binding protein binding ATP binding protein kinase A catalytic subunit binding tau protein binding tau-protein kinase activity protein serine kinase activity protease binding p53 binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding dynactin binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding dynein complex binding scaffold protein binding protein serine/threonine kinase binding integrin binding ionotropic glutamate receptor binding
          Process(es)
          epithelial to mesenchymal transition positive regulation of cell-matrix adhesion heart valve development glycogen metabolic process protein phosphorylation ER overload response mitochondrion organization meiosis I circadian rhythm insulin receptor signaling pathway negative regulation of signal transduction positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition positive regulation of protein processing regulation of neuron projection development Wnt signaling pathway peptidyl-serine phosphorylation viral protein processing hippocampus development establishment of cell polarity maintenance of cell polarity cell differentiation negative regulation of cell migration regulation of axon extension neuron projection development negative regulation of protein complex assembly positive regulation of protein complex assembly positive regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway negative regulation of TOR signaling positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of type I interferon production regulation of protein localization regulation of microtubule-based process response to endoplasmic reticulum stress intracellular signal transduction cellular response to interleukin-3 regulation of circadian rhythm regulation of apoptotic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process protein kinase B signaling positive regulation of neuron apoptotic process positive regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of glycogen biosynthetic process positive regulation of cilium assembly positive regulation of protein catabolic process negative regulation of smoothened signaling pathway protein autophosphorylation regulation of protein export from nucleus positive regulation of protein export from nucleus regulation of dendrite morphogenesis regulation of axonogenesis canonical Wnt signaling pathway excitatory postsynaptic potential intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress regulation of microtubule cytoskeleton organization negative regulation of calcineurin-NFAT signaling cascade superior temporal gyrus development cellular response to retinoic acid cellular response to glucocorticoid stimulus negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand presynaptic modulation of chemical synaptic transmission neuron projection organization regulation of microtubule anchoring at centrosome beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of substrate adhesion-dependent cell spreading regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of long-term synaptic potentiation positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to cilium negative regulation of TORC2 signaling negative regulation of dopaminergic neuron differentiation cellular response to beta-amyloid negative regulation of protein localization to centrosome positive regulation of protein localization to centrosome positive regulation of establishment of protein localization negative regulation of type B pancreatic cell development negative regulation of glycogen (starch) synthase activity positive regulation of cardiac muscle cell differentiation negative regulation of mesenchymal stem cell differentiation regulation of cell growth regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation cell migration cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation positive regulation of heart contraction positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of developmental process regulation of striated muscle cell differentiation negative regulation of cell growth involved in cardiac muscle cell development cellular response to lithium ion positive regulation of adrenergic receptor signaling pathway positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway autosome genomic imprinting regulation of mitophagy positive regulation of beta-amyloid formation positive regulation of establishment of protein localization to mitochondrion negative regulation of dendrite development G1/S transition of mitotic cell cycle re-entry into mitotic cell cycle cytoplasmic translational initiation protein export from nucleus cytoskeleton organization establishment or maintenance of cell polarity regulation of mitotic cell cycle axonogenesis myoblast fusion organ morphogenesis response to zinc ion positive regulation of mitochondrial membrane potential negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation phosphorylation positive regulation of cell migration SCF-dependent proteasomal ubiquitin-dependent protein catabolic process negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of osteoblast proliferation negative regulation of smooth muscle cell apoptotic process protein localization to microtubule cellular response to hepatocyte growth factor stimulus hepatic stellate cell activation TORC2 signaling positive regulation of apoptotic process negative regulation of MAP kinase activity fat cell differentiation regulation of osteoblast differentiation positive regulation of osteoclast differentiation positive regulation of translation positive regulation of axon extension negative regulation of synaptic growth at neuromuscular junction positive regulation of synaptic growth at neuromuscular junction bone remodeling regulation of neuronal synaptic plasticity positive regulation of smooth muscle cell proliferation negative regulation of dendrite morphogenesis negative regulation of nitric-oxide synthase activity positive regulation of protein kinase B signaling cell growth involved in cardiac muscle cell development cellular response to mechanical stimulus positive regulation of osteoclast proliferation hepatocyte apoptotic process regulation of neurotransmitter receptor localization to postsynaptic specialization membrane regulation of modification of postsynaptic structure response to methamphetamine hydrochloride conditioned place preference negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of excitatory postsynaptic potential positive regulation of DNA biosynthetic process positive regulation of stem cell differentiation negative regulation of neuron migration
          It has to be done as per old AB suggested Products section.

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