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Invitrogen

Phospho-SMAD3 (Ser423, Ser425) Recombinant Superclonal™ Antibody (3HCLC)

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Phospho-SMAD3 (Ser423, Ser425) Recombinant Superclonal™ Antibody (3HCLC)

Product Details

711324

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

3HCLC

Immunogen

Peptide corresponding to Human SMAD3 (aa 418-425)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633005

Product Specific Information

This antibody is predicted to react with Monkey, Sheep, Rat and Pig

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZP586N0721; DKFZp686J10186; JV15-2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; MGC60396; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; unnamed protein product

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Gene Aliases: hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; SMAD3

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UniProt ID: (Human) P84022

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Entrez Gene ID: (Human) 4088

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding collagen binding beta-catenin binding zinc ion binding ligand-dependent nuclear receptor binding DEAD/H-box RNA helicase binding enzyme binding protein kinase binding phosphatase binding chromatin DNA binding ubiquitin protein ligase binding mineralocorticoid receptor binding sterol response element binding glucocorticoid receptor binding identical protein binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding metal ion binding RNA polymerase II sequence-specific DNA binding transcription factor binding co-SMAD binding I-SMAD binding R-SMAD binding DNA-binding transcription factor binding promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade skeletal system development osteoblast differentiation ureteric bud development response to hypoxia in utero embryonic development mesoderm formation somitogenesis release of cytochrome c from mitochondria liver development heart looping osteoblast development immune system development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process immune response transforming growth factor beta receptor signaling pathway JNK cascade gastrulation endoderm development cell proliferation negative regulation of cell proliferation anatomical structure morphogenesis embryonic pattern specification positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial to mesenchymal transition regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression cell differentiation negative regulation of ossification negative regulation of cell growth adrenal gland development positive regulation of cell migration positive regulation of bone mineralization thyroid gland development primary miRNA processing positive regulation of chondrocyte differentiation developmental process positive regulation of interleukin-1 beta production regulation of transforming growth factor beta2 production positive regulation of transforming growth factor beta3 production activin receptor signaling pathway negative regulation of osteoblast proliferation nodal signaling pathway wound healing T cell activation negative regulation of protein catabolic process positive regulation of protein import into nucleus negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process cell-cell junction organization positive regulation of nitric oxide biosynthetic process negative regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis developmental growth embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response protein stabilization positive regulation of positive chemotaxis negative regulation of cytosolic calcium ion concentration positive regulation of stress fiber assembly regulation of mitochondrial membrane potential positive regulation of focal adhesion assembly pericardium development transdifferentiation positive regulation of SMAD protein import into nucleus SMAD protein signal transduction negative regulation of wound healing trophoblast cell migration negative regulation of lung blood pressure lens fiber cell differentiation cellular response to glucose stimulus cellular response to growth factor stimulus response to transforming growth factor beta cellular response to transforming growth factor beta stimulus positive regulation of canonical Wnt signaling pathway apoptotic signaling pathway extrinsic apoptotic signaling pathway cellular response to virus transforming growth factor beta receptor superfamily signaling pathway positive regulation of extracellular matrix assembly regulation of miRNA transcription negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cardiac muscle hypertrophy in response to stress response to angiotensin
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