Search
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
NME1 Antibody (711457) in ICC/IF
For immunofluorescence analysis, SK-OV-3 cells were fixed and permeabilized for detection of endogenous NME1 using Anti- NME1 Recombinant Rabbit Superclonal™ Antibody (Product # 711457, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of NME1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
711457
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
11HCLC
Immunogen
Protein corresponding to Human NME1 (1-152)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Pig and Bovine
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; expressed in non-metastatic cells 1, protein; farnesyl diphosphate kinase NME1; Granzyme A activated DNase (GAAD); granzyme A-activated DNase; GZMA activated DNase; histidine protein kinase NDK; histidine protein kinase NME1; included; metastasis inhibition factor NM23; NDK A; NDP kinase A; NM23H1; NM23H1B; NME1; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; non-specific serine/threonine protein kinase NME1; nucleoside diphoshate kinase 1; nucleoside-diphosphate kinase 1; nucleotide diphosphate kinase; putative 3'-5'-DNA exonuclease NDK1; putative granzyme A-activated DNA endonuclease; tumor metastatic process-associated protein; tumor metastatic process-associated protein NM23; unnamed protein product
Gene Aliases: AWD; GAAD; NB; NBS; NDK1; NDKA; NDPK A; NDPK-A; NDPKA; NM23; NM23-H1; NM23-M1; NM23A
UniProt ID: (Human) P15531, (Mouse) P15532
Entrez Gene ID: (Human) 4830, (Mouse) 18102
magnesium ion binding
DNA binding
RNA binding
endodeoxyribonuclease activity
deoxyribonuclease activity
nucleoside diphosphate kinase activity
protein histidine kinase activity
protein serine/threonine kinase activity
protein binding
3'-5'-exodeoxyribonuclease activity
kinase activity
phosphotransferase activity, phosphate group as acceptor
GDP binding
identical protein binding
ribosomal small subunit binding
ADP binding
farnesyl diphosphate kinase activity
protein serine kinase activity
coenzyme A binding
succinyl-CoA binding
acetyl-CoA binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding
intermediate filament binding
enzyme binding
protein kinase binding
gamma-tubulin binding
negative regulation of myeloid leukocyte differentiation
GTP biosynthetic process
UTP biosynthetic process
dTMP biosynthetic process
CTP biosynthetic process
DNA catabolic process
apoptotic DNA fragmentation
isoprenoid metabolic process
nucleoside phosphate metabolic process
lactation
nucleoside diphosphate metabolic process
nucleoside triphosphate biosynthetic process
negative regulation of gene expression
positive regulation of neuron projection development
mammary gland development
protein hexamerization
regulation of fatty acid biosynthetic process
ITP biosynthetic process
acetyl-CoA catabolic process
positive regulation of epithelial cell proliferation
purine-containing compound metabolic process
base-excision repair
negative regulation of cell proliferation
granzyme-mediated apoptotic signaling pathway
regulation of apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support