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FIGURE: 1 / 3
PRMT4 Antibody (711526) in WB
Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), HCT 116 (Lane 4) and HT-29 (Lane 5). The blot was probed with Anti-PRMT4 Recombinant Rabbit Superclonal™ Antibody (Product # 711526, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 65 kDa band corresponding to CARM1 was observed across the cell lines tested.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
711526
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
2HCLC
Immunogen
Protein corresponding to human PRMT4 [aa220-aa482]
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Mouse, Rat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: protein arginine N-methyltransferase 4
Gene Aliases: PRMT4
UniProt ID: (Human) Q86X55
Entrez Gene ID: (Human) 10498
transcription regulatory region sequence-specific DNA binding
transcription coactivator activity
protein binding
beta-catenin binding
protein methyltransferase activity
protein-arginine N-methyltransferase activity
protein-arginine omega-N asymmetric methyltransferase activity
histone methyltransferase activity (H3-R17 specific)
histone methyltransferase activity
histone methyltransferase activity (H3-R2 specific)
DNA-binding transcription factor binding
chromatin remodeling
regulation of transcription, DNA-templated
positive regulation of cell proliferation
regulation of intracellular estrogen receptor signaling pathway
positive regulation of fat cell differentiation
positive regulation of transcription from RNA polymerase I promoter
response to cAMP
replication fork reversal
positive regulation of epithelial cell apoptotic process
negative regulation of dendrite development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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