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Invitrogen

Phospho-PKA alpha/beta (Thr197) Recombinant Superclonal™ Antibody (6HCLC)

View all (7) PKA alpha/beta antibodies

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Product Details

711722

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

6HCLC

Immunogen

Peptide corresponding to Human PRKACA, PRKACB (aa Chemically synthesized phosphopeptide derived from a region of human PKA around 197)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633194

Product Specific Information

This antibody is predicted to react with Monkey, Rat, Mouse, Bovine and Pig

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-alpha-s; alternatively spliced isoform; cAMP-dependent protein kinase C beta; Cbeta2 PKA; PKA C-alpha; protein kinase A catalytic subunit; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; protein kinase, cAMP-dependent, catalytic, beta; sperm cAMP-dependent protein kinase catalytic subunit; unnamed protein product

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Gene Aliases: CAFD1; CAFD2; CbPKA; PKA C-beta; PKACA; PKACB; PKCD; PPNAD4

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UniProt ID: (Human) P17612, (Human) P22694, (Mouse) P05132, (Mouse) P68181

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Entrez Gene ID: (Human) 5566, (Human) 5567, (Mouse) 18747, (Mouse) 18749

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Function(s)
magnesium ion binding protein kinase activity protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding potassium channel inhibitor activity protein kinase binding protein domain specific binding manganese ion binding protein kinase A regulatory subunit binding channel activator activity protein serine kinase activity histone H1-4S35 kinase activity small GTPase binding ubiquitin protein ligase binding macromolecular complex binding cyclic nucleotide-dependent protein kinase activity
Process(es)
neural tube closure protein phosphorylation adenylate cyclase-modulating G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway cellular response to nutrient levels TORC1 signaling negative regulation of smoothened signaling pathway negative regulation of meiotic cell cycle regulation of protein processing negative regulation of TORC1 signaling mesoderm formation chromatin remodeling mRNA processing adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway positive regulation of cell proliferation positive regulation of triglyceride catabolic process peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cellular potassium ion homeostasis cellular response to heat insulin secretion involved in cellular response to glucose stimulus interleukin-2-mediated signaling pathway regulation of cellular respiration positive regulation of cholesterol biosynthetic process regulation of osteoblast differentiation positive regulation of gluconeogenesis negative regulation of glycolytic process protein autophosphorylation positive regulation of protein export from nucleus sperm capacitation spontaneous exocytosis of neurotransmitter positive regulation of phagocytosis modulation of synaptic transmission regulation of cell cycle regulation of synaptic transmission, glutamatergic regulation of proteasomal protein catabolic process cellular response to cold regulation of microtubule cytoskeleton organization cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus postsynaptic modulation of chemical synaptic transmission negative regulation of protein localization to chromatin cAMP/PKA signal transduction positive regulation of protein localization to plasma membrane negative regulation of glycolytic process through fructose-6-phosphate protein localization to lipid particle regulation of bicellular tight junction assembly regulation of heart rate renal water homeostasis regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of macroautophagy cytokine-mediated signaling pathway positive regulation of insulin secretion negative regulation of interleukin-2 production high-density lipoprotein particle assembly mitochondrial protein catabolic process positive regulation of calcium-mediated signaling regulation of cardiac muscle contraction cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction vascular endothelial cell response to laminar fluid shear stress regulation of cardiac conduction signal transduction
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