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Invitrogen
TCF2 Polyclonal Antibody
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FIGURE: 1 / 5
TCF2 Antibody (720259) in ICC/IF
For immunofluorescence analysis, Hep G2 cells were fixed and permeabilized for detection of endogenous HNF1b using HNF1b Rabbit Polyclonal Antibody (Product# 720259, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HNF1b protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) repr... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
720259
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to Human HNF1b (aa 511:528, 191:208)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2-7.4, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Horse and Cat.
Target Information
Hepatocyte Nuclear Factor 1 (HNF1, Homeoprotein LFB3, Transcription factor 2, TCF2, Variant hepatic nuclear factor) is a member of the homeodomain-containing superfamily of transcription factors. It is a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. HNF1has been shown to function in nephron development and regulates development of the embryonic pancreas. Mutations in the gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of the gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HNF1 beta A; homeoprotein LFB3; TCF2; Tcf2-splice variant; transcription factor; transcription factor 2; Transcription factor 2, hepatic; unnamed protein product; variant hepatic nuclear factor 1
Gene Aliases: ADTKD3; FJHN; HNF-1-beta; HNF-1B; HNF-1Beta; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; RCAD; T2D; TCF-2; TCF2; VHNF1
UniProt ID: (Human) P35680, (Mouse) P27889, (Rat) P23899
Entrez Gene ID: (Human) 6928, (Mouse) 21410, (Rat) 25640
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription cofactor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
protein homodimerization activity
promoter-specific chromatin binding
sequence-specific DNA binding
macromolecular complex binding
double-stranded DNA binding
kidney development
liver development
regulation of transcription from RNA polymerase II promoter
insulin secretion
pancreas development
endocrine pancreas development
regulation of pronephros size
pronephric nephron tubule development
positive regulation of transcription, DNA-templated
animal organ development
system development
pronephros development
genitalia development
positive regulation of transcription initiation from RNA polymerase II promoter
negative regulation of transcription from RNA polymerase II promoter
formation of primary germ layer
endoderm formation
endodermal cell fate specification
blastocyst development
inner cell mass cell differentiation
Notch signaling pathway
endoderm development
response to glucose
anterior/posterior pattern specification
gene expression
positive regulation of gene expression
regulation of Wnt signaling pathway
hindbrain development
circadian regulation of gene expression
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract morphogenesis
embryonic morphogenesis
branching morphogenesis of an epithelial tube
epithelial cell proliferation
epithelium development
ureteric bud elongation
kidney morphogenesis
hepatoblast differentiation
protein-DNA complex assembly
regulation of branch elongation involved in ureteric bud branching
mesonephric tubule development
nephric duct development
mesonephric duct development
nephric duct formation
mesonephric duct formation
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
response to xenobiotic stimulus
response to carbohydrate
hepatocyte differentiation
response to biphenyl
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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