C6 cells expressing ÃŸ-galactosidase were grown in 96-well plates. Cells were lysed and assayed using Thermo Scientific M-PER Mammalian Protein Extraction Reagent and Pierce ÃŸ-Gal Assay Reagent or corresponding Supplier X reagents. Reactions were stopped with 150ÂµL of Stop Solution provided in each kit, and absorbances were measured at 405nm using a plate-reading spectrophotometer.
Comparison of beta-galactosidase assay kit protocols
|Description:||Pierce beta-Galactosidase Assay Reagent|
|Substrate Type:||Beta-Gal Substrate|
|Compatible Cells:||Mammalian Cells|
|Label Type:||Enzyme Labeled|
|Substrate Properties:||Chemical Substrate|
|For Use With (Equipment):||Microplate Reader|