Thermo Scientific™

beta-Galactosidase Assay Reagent

Catalog number: 75705
Thermo Scientific™

beta-Galactosidase Assay Reagent

Catalog number: 75705
Catalog Number
75705
Unit Size
25 mL
Price (USD)
137.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
3 x 25 mL
Price (USD)
295.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
7570525 mL
137.00
Your Price:
***
757103 x 25 mL
295.00
Your Price:
***
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The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria

Related Products
Mammalian beta-Galactosidase Assay Kit
beta-Galactosidase Assay Stop Solution
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay
Reporter Enzyme
Detection Method
Colorimetric
Form
Liquid
For Use With (Application)
Absorbance
Product Line
Pierce™
Quantity
25 mL
Substrate
Beta-Gal Substrate
Substrate Properties
Chemical Substrate
Target Enzyme
Beta-Galactosidase
Format
96-well Plate
For Use With (Equipment)
Microplate Reader
Label or Dye
Enzyme Labeled
Product Type
Pierce beta-Galactosidase Assay Reagent

Contents & Storage

Store at -20°C.

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