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Invitrogen

CD36 Monoclonal Antibody (HM36), Super Bright™ 780, eBioscience™

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1 Reference
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CD36 Monoclonal Antibody (HM36), Super Bright™ 780, eBioscience™

Product Details

78-0362-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HM36

Immunogen

CHO cells transfected with full-length mouse CD36
3D Epitope / Immunogen

Conjugate

Super Bright™ 780 Super Bright™ 780 Super Bright™ 780

Excitation/Emission Max

413/780 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2734973

Product Specific Information

Description: This HM36 antibody recognizes mouse CD36, also known as scavenger receptor BIII.

Applications Reported: This HM36 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HM36 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: Cd36; CD36 antigen; fatty acid translocase; glycoprotein IIIb; GPIIIB; PAS IV; PAS-4; platelet glycoprotein IV; SR-B3; unnamed protein product

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Gene Aliases: FAT; GPIV; Scarb3

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UniProt ID: (Mouse) Q08857

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Entrez Gene ID: (Mouse) 12491

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Function(s)
beta-amyloid binding low-density lipoprotein receptor activity scavenger receptor activity long-chain fatty acid transporter activity protein binding high-density lipoprotein particle binding lipid binding short-chain fatty acid uptake transporter activity low-density lipoprotein particle binding Toll-like receptor binding signaling receptor activity cargo receptor activity macromolecular complex binding transforming growth factor beta binding thrombospondin receptor activity oleic acid binding lipoteichoic acid receptor activity lipoprotein particle binding oxidised low-density lipoprotein particle receptor activity oleate transmembrane transporter activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter long-chain fatty acid metabolic process positive regulation of cytokine production positive regulation of cell-matrix adhesion negative regulation of systemic arterial blood pressure fatty acid metabolic process triglyceride metabolic process lipid transport receptor-mediated endocytosis phagocytosis, recognition inflammatory response cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration response to mechanical stimulus response to bacterium positive regulation of gene expression negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of cholesterol storage fatty acid transport long-chain fatty acid transport plasma membrane long-chain fatty acid transport short-chain fatty acid transport negative regulation of angiogenesis fatty acid oxidation lipid storage positive regulation of blood coagulation intestinal cholesterol absorption cholesterol transport receptor internalization positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production response to lipid regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor 4 signaling pathway triglyceride transport chylomicron assembly plasma lipoprotein particle clearance low-density lipoprotein particle clearance cellular response to oxidative stress response to stilbenoid nitric oxide-cGMP-mediated signaling pathway negative regulation of protein import into nucleus eating behavior lipoprotein transport positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of protein complex assembly apoptotic cell clearance positive regulation of MAPK cascade positive regulation of skeletal muscle tissue regeneration long-chain fatty acid import positive regulation of nitric oxide biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of angiogenesis positive regulation of phagocytosis defense response to Gram-positive bacterium intestinal absorption sensory perception of taste low-density lipoprotein particle mediated signaling positive regulation of phagocytosis, engulfment positive regulation of macrophage cytokine production positive regulation of stress-activated protein kinase signaling cascade positive regulation of ERK1 and ERK2 cascade cholesterol import response to fatty acid response to linoleic acid cellular response to bacterial lipopeptide cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to low-density lipoprotein particle stimulus cellular response to hydroperoxide cellular response to diacyl bacterial lipopeptide positive regulation of triglyceride metabolic process energy homeostasis cell-cell adhesion regulation of action potential positive regulation of cold-induced thermogenesis cellular response to oxidised low-density lipoprotein particle stimulus oxidised low-density lipoprotein particle clearance amyloid-beta clearance by cellular catabolic process positive regulation of NLRP3 inflammasome complex assembly positive regulation of protein localization to plasma membrane positive regulation of reactive oxygen species biosynthetic process cellular response to beta-amyloid amyloid fibril formation lipid transport across blood brain barrier regulation of removal of superoxide radicals positive regulation of blood microparticle formation positive regulation of reactive oxygen species metabolic process
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