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View additional product information for GeneArt™ Custom DNA Fragment 751-1000 bp - FAQs (815030DE)
10 product FAQs found
The kit and custom services offer different advantages. Please click here (http://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis-product-selection-guide.html) to view a selection guide comparing the technologies, including deliverables and production time, to determine what is best for you.
GeneArt Strings fragments are PCR amplicons of assembled oligonucleotides, ready for screening to identify the correct clone. Strings fragments are bulk sequence-controlled as part of the QC process to help ensure that your desired sequence is present in the fragment pool.
To identify a correct clone >90% of the time, we recommend using these screening guidelines:
•Strings fragments up to 1 kb: Sequence 2 to 4 full-length clones
•Strings fragments 1-2 kb: Sequence 3 to 5 full-length clones
•Strings fragments 2-3 kb: Sequence 4 to 8 full-length clones
No. GeneArt Strings DNA fragments are limited to 100 to 3,000 bp. If your sequence is longer, you need to order it as gene synthesis or assemble your gene from two or more GeneArt Strings DNA fragments. In addition, the GeneArt Strings DNA fragments manufacturing process is streamlined to quickly and cost-effectively provide you with your gene product. If your sequence is complex, you may receive a message that it cannot be produced as a Strings DNA fragment due to high complexity. In that case, we recommend modifying your sequence to match the production criteria (given below) or ordering your gene as gene synthesis. In many cases, optimizing your sequence using the portal function enables manufacturing with the GeneArt Strings fragments process. If it is still not able to be produced, you can manually edit the sequence to enable production.
Important production criteria are:
• GC content from 20 to 80% (in peaks, not overall content)
• No long secondary structures or sequence repetitions
• No A/T stretches >25 bp nor G/C stretches >20 bp
Yes, it is possible to directly assemble two GeneArt Strings fragments without pre-cloning. We offer several technologies for seamless assembly, e.g., GeneArt Type IIs Assembly or GeneArt Seamless Cloning and Assembly. Depending on the sequence length and the number of subfragments you want to assemble, the screening effort to find a correct clone can be high. Therefore, we generally recommend pre-cloning the subfragments to limit the screening effort required to find a correct clone after assembly.
No, subcloning and assembly services are not available for GeneArt Strings DNA fragments, as they are designed to offer you the fastest and most affordable way to get access to your genes. If you do not want to clone the gene yourself, we recommend that you order full gene synthesis service.
Yes. We recommend using the GeneArt web portal for ordering, where you can use the free gene editing and optimization functions to adjust the sequence to meet your needs. After editing and optimization, please check again that the final sequences of the fragments are exactly as needed for further processing in your lab (e.g., potential homologous overlaps of the fragments or restriction sites and buffer bases needed for cloning).
No. GeneArt Strings DNA fragments from 150 to 1,000 bp are produced in 5 business days and GeneArt Strings DNA fragments from 1,000 to 3,000 bp are produced in 8 business days. Depending on the nature of the sequence, production time can vary.
GeneArt Strings DNA fragments are shipped dried, ready for resuspension and cloning. Before using, we recommend spinning down the tube contents, adding some water to the bottom of the tube to get the desired concentration, and incubating for at least 1 hour at room temperature (alternatively, incubate at 4°C overnight). Subsequently, we recommend resuspending the fragments carefully and using immediately. For longer storage, resuspended GeneArt Strings DNA fragments should be dispensed into aliquots and frozen at -20°C. Please avoid freeze-thaw cycles.
GeneArt Strings DNA fragments are PCR amplicons of assembled oligonucleotides; an intermediate product of the gene synthesis production process. This process results in a pool of fragments, and cloning and screening need to be carried out to identify the correct clone. To ensure that correct fragments are present in the pool, GeneArt Strings fragments are bulk sequence-controlled before shipment.
GeneArt Strings DNA fragments are custom-made, uncloned, double-stranded linear DNA fragments, available in lengths from 150 to 3,000 base pairs (bp), assembled from synthetic oligonucleotides using the same process developed for GeneArt high-quality gene synthesis. GeneArt Strings fragments can be used to quickly clone your gene of interest. At least 200 ng of GeneArt Strings DNA fragments are produced within 5 business days (up to 1,000 bp) or 8 business days (from 1,000 to 3,000 bp). GeneArt Strings DNA fragments can be designed, sequence-optimized, and ordered online and are a cost-effective and smart alternative to clone your expression plasmid. Please refer to thermofisher.com/strings for further details.