SuperSignal™ Molecular Weight Protein Ladder

Catalog number: 84785

Thermo Scientific™  Related applications: Protein Assays and Analysis | Protein Gel Electrophoresis

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Description

Thermo Scientific SuperSignal Molecular Weight Protein Ladder (20 to 150K) are a ready-to-use mix of recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems.

Features of SuperSignal Molecular Weight Protein Ladder:

Ready-to-use—liquid format is ready to load and requires no boiling
Direct visualization—see marker bands with gel stains and blotting detection reagents
Consistent—even band development regardless of detection method
Stable—store long term at -20°C, three months at 4°C or one month at ambient

SuperSignal Molecular Weight Protein Ladder contain ready-to-use stabilized mixtures of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. The MW marker mixes also contain a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers. The standards are compatible with nitrocellulose and PVDF membranes, multiple blocking buffers and many other detection methods.

SuperSignal Molecular Weight Protein Ladders provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent, fluorescent, chromogenic or other detection systems. Two versions of the MW marker are offered. The regular formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. The enhanced ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1/50,000 (25 ng/mL) to 1/100,000 (10 ng/mL) dilution of secondary antibody with SuperSignal West Pico Chemiluminescent substrate.

SuperSignal Molecular Weight Protein Ladders have flexible storage conditions depending on how often they are used. For intermittent use, store the molecular weight marker mix at -20°C. For labs performing gel electrophoresis on a routine basis, the protein standards can be kept at ambient temperature on the lab bench for one month or stored at 4°C for three months.

Related Products
SuperSignal™ Enhanced Molecular Weight Protein Ladder
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Stain Type: Unstained
System: SDS-PAGE
Product Size: 250 µL

Contents & storage

Upon receipt store at -20°C. Product is shipped with an ice pack.

Documents

Manuals & protocols