Thermo Scientific™

SuperSignal™ Enhanced Molecular Weight Protein Ladder

Catalog number: 84786
Thermo Scientific™

SuperSignal™ Enhanced Molecular Weight Protein Ladder

Catalog number: 84786
Catalog Number
84786
Unit Size
250 µL
Price (USD)
Price: 299.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
84786250 µL
Price: 299.00
Your Price:
-
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Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).

Two versions of the MW marker are offered. This formulation should be used in applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody. The SuperSignal Molecular Weight Protein ladder formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
User defined detection system
Number of Markers
8
Product Type Specs
Protein Ladder
Ready to Load
Yes
Size Range
20 to 150 kDa
Stain Type
Unstained
Gel Compatibility
Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight
150, 100, 80, 60, 50, 40, 30, 20 kDa
Product Line
SuperSignal™
Quantity
250 µL
Shipping Condition
Wet Ice
System Type
Western Blotting, SDS-PAGE

Contents & Storage

Contents: one vial of 250 µL

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient