Pierce™ Albumin Depletion Kit

Catalog number: 85160

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Description

Pierce Albumin Depletion Resin is supplied as a 50% slurry. Dispense 200 µL of slurry into the supplied microcentrifuge spin columns to obtain 100 µL of settled beads for the standard protocol. Each aliquot of resin can be used to process 10 to 50 µL of serum sample in a single reaction. Processed samples are ready for immediate downstream analysis by electrophoresis or MS applications. The ease and versatility offered by the slurry format make this kit ideal for single- or multi-sample processing with the microcentrifuge spin columns supplied in the kit.

Features of the Albumin Depletion Kit:

Complete kit—includes optimized buffer and microcentrifuge spin columns to remove albumin quickly and conveniently from 10 to 50 µL samples
Easy-to-dispense slurry—improves performance, eases processing of multiple samples, and can be adapted to larger or smaller columns or 96-well filter plates
Workflow compatible—removing excess albumin facilitates MS or electrophoresis gel analysis of low-abundance serum proteins

Includes:
• Resin slurry, binding-wash buffer, and microcentrifuge spin columns

Human serum albumin (HSA) often accounts for greater than 60% of the total protein present in serum samples and can have a concentration of approximately 40 mg/mL. The high concentration of albumin hinders research, obscuring the detection of many proteins of biological interest. Traditionally, researchers have produced albumin-free samples using chromatography methods involving multiple purification steps. In addition to involving lengthy and tedious procedures, these purification steps also tend to give low protein yields. The Pierce Albumin Depletion Kit was developed to take advantage of the Cibacron* Blue Dye albumin binding properties.

The kit is optimized to bind human, porcine and sheep albumin from serum samples. With a modification to the protocol, albumin from bovine, calf, goat and rat can be removed with this method. The kit is not effective for removal of mouse albumin.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 24 reactions
Final Product: Protein
Workflow Step: Sample Enrichment, Sample Clean Up
Detection Method: Mass Spectrometry
Starting Material: Serum, Plasma
For Use With (Equipment): Mass Spectrometer, Microcentrifuge

Contents & storage

Sufficient For: Removing albumin from 24 human serum samples, 10 to 50 µL each
• Pierce Albumin Depletion Resin, 5 mL
• Binding/Wash Buffer, 11 mL
• Pierce Spin Columns, 24 columns

Store at 4°C.

Documents

Manuals & protocols

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