Thermo Scientific™

Pierce™ C18 Spin Tips & Columns

Catalog number: 87784
Thermo Scientific™

Pierce™ C18 Spin Tips & Columns

Catalog number: 87784
Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spectra. Sample clean-up with C18 resins significantly improves signal-to-noise ratios and sequence coverage by removing MS-incompatible salts and detergents commonly used in protein or peptide preparation. The optimized protocol for the Pierce C-18 Spin Columns effectively removes contaminants and elutes peptides in MS-compatible solutions.
 
Catalog Number
87784
Unit Size
96 tips
Product Type
Pipette Tip
Quantity
96 x 100 µL Tips
Price (USD)
Price: 342.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionPierce C-18 Tips, 100 µL bed
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypePipette Tip
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatTip
Workflow StepSample Clean Up
Quantity96 x 100 µL Tips
Unit Size96 tips
Catalog Number
Unit Size
25 columns
Product Type
Column
Quantity
25 Columns
Price (USD)
Price: 150.00
Your Price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeColumn
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
Workflow StepSample Clean Up
Quantity25 Columns
Unit Size25 columns
Catalog Number
Unit Size
50 columns
Product Type
Column
Quantity
50 Columns
Price (USD)
Price: 263.00
Your Price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeColumn
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatSpin Column
Workflow StepSample Clean Up
Quantity50 Columns
Unit Size50 columns
Catalog Number
Unit Size
96 tips
Product Type
Spin Tip
Quantity
96 Tips
Price (USD)
Price: 312.00
Your Price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeSpin Tip
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatSolid
Workflow StepSample Clean Up
Quantity96 Tips
Unit Size96 tips
Catalog Number
Unit Size
96 tips
Product Type
Pipette Tip
Quantity
96 x 10 µL Tips
Price (USD)
Price: 299.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionPierce C-18 Tips, 10 µL bed
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypePipette Tip
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatTip
Workflow StepSample Clean Up
Quantity96 x 10 µL Tips
Unit Size96 tips
Showing 5 of 5
Catalog NumberSpecificationsUnit SizeProduct TypeQuantityPrice (USD)AvailabilityQuantity
87784Full specifications
96 tipsPipette Tip96 x 100 µL Tips
Price: 342.00
Your Price:
-
DescriptionPierce C-18 Tips, 100 µL bed
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypePipette Tip
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatTip
Workflow StepSample Clean Up
Quantity96 x 100 µL Tips
Unit Size96 tips
89870Full specifications
25 columnsColumn25 Columns
Price: 150.00
Your Price:
-
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeColumn
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
Workflow StepSample Clean Up
Quantity25 Columns
Unit Size25 columns
89873Full specifications
50 columnsColumn50 Columns
Price: 263.00
Your Price:
-
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeColumn
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatSpin Column
Workflow StepSample Clean Up
Quantity50 Columns
Unit Size50 columns
84850Full specifications
96 tipsSpin Tip96 Tips
Price: 312.00
Your Price:
-
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeSpin Tip
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatSolid
Workflow StepSample Clean Up
Quantity96 Tips
Unit Size96 tips
87782Full specifications
96 tipsPipette Tip96 x 10 µL Tips
Price: 299.00
Your Price:
-
DescriptionPierce C-18 Tips, 10 µL bed
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypePipette Tip
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatTip
Workflow StepSample Clean Up
Quantity96 x 10 µL Tips
Unit Size96 tips
Showing 5 of 5
Pierce C18 Spin Columns
Thermo Scientific Pierce C18 Spin Columns are ready-to-use centrifuge columns of porous C18 reverse-phase resin for peptide sample preparation for MS and other analysis methods. The protocol has been optimized to efficiently remove interfering contaminants and release peptides in MS-compatible solutions. Columns can be used effectively for processing peptides derived from 10 ng to 30 µg of protein. Sensitivity and detection limits are dependent on the downstream application.

Efficient—effectively removes interfering contaminants to improve spectral quality and is suitable for a variety of reverse-phase-compatible contaminants
Flexible—works with a wide variety of load volumes and concentrations; no need to reduce sample volume before application
Convenient—spin column format requires no special equipment to process multiple samples simultaneously
Robust—special C18 resin allows excellent recovery percentages, even at low (sub-picomole) sample loads

Pierce C18 Spin Tips
Thermo Scientific Pierce C18 Spin Tips enable fast and efficient capture, concentration, desalting, and elution of up to 10 µg of peptides per 20 µL of sample with volumes ranging from 10 to 100 µL. The five-step procedure is simple and requires less than five minutes to process protein digests, strong cation exchange fractions, and other protein and peptide samples for mass spectrometric (MS) analyses. The Pierce C18 Spin Tips offer excellent flow properties with a high-efficiency monolithic C18 sorbent for fast wetting, loading, washing, and eluting.

Efficient—effectively removes interfering contaminants to improve spectral quality and is suitable for a variety of reverse-phase-compatible contaminants
Rapid—C18 fast-flow tips have low resistance and improved flow
Convenient—spin tips are provided with tip adaptor for easy centrifugation
Cleaner sample—device filters particulates that can cause autosampler and column clogging

Applications
• Clean-up of peptides after protein digestion
• Peptide sample concentration and desalting
• Sample preparation for MALDI peptide analysis and proteomics workflows

For Research Use Only. Not for use in diagnostic procedures.

Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) are essential for studying biological compounds because of the high sensitivity and mass accuracy they provide. However, many of the buffers and compounds common to biological samples interfere with both MALDI-MS and ESI-MS, resulting in reduced sensitivity and poor-quality spectra. Pierce C-18 Spin Columns remove interfering contaminants and release peptides in MS-compatible solutions, providing an effective tool that aids in the MS examination of post-translational modifications or for the identification of proteins by peptide mapping.

Contents & Storage

Store at room temperature.